公开(公告)号：EP3828274A1

公开(公告)日：2021-06-02

申请号：EP19841320.5

申请日：2019-07-25

申请人： NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY

发明人： MASUTA, Chikara ,  INUKAI, Tsuyoshi ,  MATSUNAGA, Wataru ,  ISODA, Reika ,  MATSUMURA, Takeshi ,  ATSUMI, Go

IPC分类号： C12N15/113 ,  A01H3/00 ,  C12N5/10 ,  C12N15/82 ,  C12P1/00

摘要： Plants are created with desired traits by conveniently and rapidly inhibiting methylation of target DNA in plants, without using recombination technology. Scaffold RNA produced by transcription of target DNA is cleaved in an RNA-directed DNA methylation mechanism.

公开(公告)号：EP4083207A1

公开(公告)日：2022-11-02

申请号：EP20904326.4

申请日：2020-12-25

申请人： National Institute Of Advanced Industrial Science and Technology

发明人： ATSUMI, Go ,  MATSUMURA, Takeshi ,  MASUTA, Chikara ,  INUKAI, Tsuyoshi ,  MATSUNAGA, Wataru ,  ISODA, Reika

IPC分类号： C12N15/113 ,  A01H1/00

摘要： According to the present invention, methylation of a target DNA in a plant is suppressed quickly and easily without using recombinant technology, and a plant having desired traits is thereby created. In an RNA-directed DNA methylation mechanism, binding between scaffold RNA produced by transcription of the target DNA and an siRNA-AGO4 complex is inhibited.