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1.发明公开An RNA plant virus vector or portion thereof, a method of construction thereof, and a method of producing a gene derived product therefrom 失效RNA植物病毒载体或其部分，其构建方法和生产基因衍生产物的方法
公开(公告)号：EP0067553A3
公开(公告)日：1984-04-11
申请号：EP82302574
申请日：1982-05-20
CPC分类号： C12N15/8203
摘要： The method of construction of at least a portion of, an RNA vector from an RNA plant virus, e.g. tobacco mosaic virus (TMV) using as specific functional regions, at least two nucleotide sequences selected from oligonucleotides and polynucleotides of viral RNA for the purpose of producing an RNA molecule containing those nucleotide sequences essential for self-replication or those nucleotide sequences essential for replication upon co-infection with complete TMV in a plant cell or cells, and having the potential, in the plant cell or cells, to express and control the expression of genetic information inserted thereby and those regions necessary for the production and accumulation of stable vector particles in the plant or plant cells. The production of a gene-derived product in a plant cell from the said at least a portion of an RNA vector is also described.
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2.发明公开An RNA plant virus vector or portion thereof, a method of construction thereof, and a method of producing a gene derived product therefrom 失效RNA植物病毒载体或其部分，以制备过程及其制备和方法，以从中获得的基因衍生的产物。
公开(公告)号：EP0067553A2
公开(公告)日：1982-12-22
申请号：EP82302574.7
申请日：1982-05-20
IPC分类号： C12N15/00
CPC分类号： C12N15/8203
摘要： The method of construction of at least a portion of, an RNA vector from an RNA plant virus, e.g. tobacco mosaic virus (TMV) using as specific functional regions, at least two nucleotide sequences selected from oligonucleotides and polynucleotides of viral RNA for the purpose of producing an RNA molecule containing those nucleotide sequences essential for self-replication or those nucleotide sequences essential for replication upon co-infection with complete TMV in a plant cell or cells, and having the potential, in the plant cell or cells, to express and control the expression of genetic information inserted thereby and those regions necessary for the production and accumulation of stable vector particles in the plant or plant cells. The production of a gene-derived product in a plant cell from the said at least a portion of an RNA vector is also described.
摘要翻译： 结构的至少一部分的所述的方法，计划RNA载体从RNA病毒，E. G.的 使用如特定功能区域烟草花叶病毒（TMV），从寡核苷酸和病毒RNA的多核苷酸，选择用于在RNA分子产生包含自我复制或用于在复制所必需的那些核苷酸序列必需的那些核苷酸序列的目的，至少两个核苷酸序列 共感染在植物细胞或细胞和完整TMV具有在一个或多个小区必要时作稳定载体颗粒的生产和积累的电势图，表示和控制的遗传信息从而插入表达和这些区域 所述植物或植物细胞。 因此至少RNA载体的一部分中描述了从所述的植物细胞生产的基因衍生的产物。
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