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公开(公告)号：EP0109859A3
公开(公告)日：1985-09-11
申请号：EP83307139
申请日：1983-11-22
申请人： NIPPON OIL CO. LTD.
发明人： Endo, Noboru , Kojima, Ichiro , Oguchi, Yutaka
摘要： 57 A process for the separation of vitamin B 12 in pure form which comprises bringing a vitamin B 12 -containing liquid having impurities into contact with a divinylbenzene styrene copolymer resin, and causing vitamin B 12 to be adsorbed to the resin. The vitamin B 12 thus adsorbed is eluted an eluent which is an aqueous solution containing a compound selected from the group consisting of a lower ketone, a lower ester and a lower ether. The resin may be treated before elution with purified water or an aqueous low-acidity solution.
摘要翻译： 用于分离纯形式的维生素B12的方法，其包括使具有杂质的含维生素B12的液体与二乙烯基苯苯乙烯共聚物树脂接触，并使维生素B12吸附到树脂上。 将这样吸附的维生素B12洗脱洗脱液，洗脱液是含有选自下酮，低级酯和低级醚的化合物的水溶液。 可以用纯化水或低酸度水溶液洗脱之前处理树脂。
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2.发明授权Process for producing vitamin B12 by the fermentation technique, and vitamin B12-producing microorganism 失效利用发酵技术生产维生素B12的方法和维生素B12生产微生物
公开(公告)号：EP0087920B1
公开(公告)日：1987-06-24
申请号：EP83300952.5
申请日：1983-02-23
申请人： NIPPON OIL CO. LTD.
发明人： Kojima, Ichiro , Komiya, Kouji , Sato, Hiroshi , Oguchi, Yutaka
CPC分类号： C12R1/01 , C12P19/42 , Y10S435/822
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3.发明公开Process for producing vitamin B12 by the fermentation technique, and vitamin B12-producing microorganism 失效一种用于通过发酵技术和维生素B12的微生物生产维生素B12的过程。
公开(公告)号：EP0087920A1
公开(公告)日：1983-09-07
申请号：EP83300952.5
申请日：1983-02-23
申请人： NIPPON OIL CO. LTD.
发明人： Kojima, Ichiro , Komiya, Kouji , Sato, Hiroshi , Oguchi, Yutaka
CPC分类号： C12R1/01 , C12P19/42 , Y10S435/822
摘要： A process for producing vitamin B 12 by the fermentation technique which comprises cultivating a vitamin B 12 - producing microorganism belonging to the genus Propionibacterium in a culture medium containing a carbon source and a nitrogen source, and collecting vitamin B 12 accumulated in the cells of the microorganism; characterized in that
(1) cultivation is carried out while adding a base to maintain the pH at 5 to 7.5, and
(2) cultivation is carried out while adding a carbon source portionwise to the cultivation system nearly within 10 minutes of adding the base,
(3) provided that when a vitamin B 12 -producing microorganism having resistance to propionic acid is used as the microorganism, the portionwise addition of the carbon source in (2) can be omitted; and a propionic acid-resistant, vitamin B 12 -producing microorganism of the genus Proproni- bactenum.-
4.发明公开
公开(公告)号：EP0071966A2
公开(公告)日：1983-02-16
申请号：EP82107011.7
申请日：1982-08-03
申请人： NIPPON OIL CO. LTD.
CPC分类号： C12P17/182 , B01J20/26
摘要： A process for separating porphyrins, which comprises
(1) adjusting the pH of the liquid phase of a culture broth containing at least two porphyrins to about 2.5 to about 4 and collecting the resulting solid containing the porphyrins,
(2) either (a) preparing an acidic aqueous solution of said solid and adjusting the pH of its liquid phase left after removal of insoluble matter to about 6 to about 8 to form an aqueous solution containing the porphyrins, or (b) preparing an acid-containing lower alcohol solution of said solid, adjusting the pH of its liquid phase left after removal of insoluble matter, adding water in an amount at least twice the amount of the solution to precipitate lower alkyl esters of the porphyrins, collecting the precipitates and dissolving them in an organic solvent to form an organic solvent solution of the porphyrin esters,
(3) contacting the solution obtained in (a) or (b) in step (2) with a copolymer having a surface area of at least about 700 m 2 /g and derived from
(i) divinylbenzene,
(ii) styrene or its functional derivative,
(iii) an alkenyl ester of a benzenepolycarboxylic acid represented by the following formula
wherein R represents an alkenyl group having 3 to 10 carbon atoms, and n is 2 or 3,
thereby permitting adsorption of the porphyrins or their esters to the copolymer, and
(4) thereafter, eluting the copolymer to obtain fractions of the individual porphyrins or their esters; and an adsorbent for use in the above-mentioned process.-
公开(公告)号：EP0071966A3
公开(公告)日：1984-08-22
申请号：EP82107011
申请日：1982-08-03
申请人： NIPPON OIL CO. LTD.
IPC分类号： C12P17/18 , C08L25/14 , B01J20/26 , C07D487/22
CPC分类号： C12P17/182 , B01J20/26
摘要： A process for separating porphyrins, which comprises
(1) adjusting the pH of the liquid phase of a culture broth containing at least two porphyrins to about 2.5 to about 4 and collecting the resulting solid containing the porphyrins, (2) either (a) preparing an acidic aqueous solution of said solid and adjusting the pH of its liquid phase left after removal of insoluble matter to about 6 to about 8 to form an aqueous solution containing the porphyrins, or (b) preparing an acid-containing lower alcohol solution of said solid, adjusting the pH of its liquid phase left after removal of insoluble matter, adding water in an amount at least twice the amount of the solution to precipitate lower alkyl esters of the porphyrins, collecting the precipitates and dissolving them in an organic solvent to form an organic solvent solution of the porphyrin esters, (3) contacting the solution obtained in (a) or (b) in step (2) with a copolymer having a surface area of at least about 700 m 2 /g and derived from
(i) divinylbenzene, (ii) styrene or its functional derivative, (iii) an alkenyl ester of a benzenepolycarboxylic acid represented by the following formula
wherein R represents an alkenyl group having 3 to 10 carbon atoms, and n is 2 or 3,
thereby permitting adsorption of the porphyrins or their esters to the copolymer, and (4) thereafter, eluting the copolymer to obtain fractions of the individual porphyrins or their esters; and an adsorbent for use in the above-mentioned process.-
6.发明公开
公开(公告)号：EP0109859A2
公开(公告)日：1984-05-30
申请号：EP83307139.2
申请日：1983-11-22
申请人： NIPPON OIL CO. LTD.
发明人： Endo, Noboru , Kojima, Ichiro , Oguchi, Yutaka
摘要： 57 A process for the separation of vitamin B 12 in pure form which comprises bringing a vitamin B 12 -containing liquid having impurities into contact with a divinylbenzene styrene copolymer resin, and causing vitamin B 12 to be adsorbed to the resin. The vitamin B 12 thus adsorbed is eluted an eluent which is an aqueous solution containing a compound selected from the group consisting of a lower ketone, a lower ester and a lower ether. The resin may be treated before elution with purified water or an aqueous low-acidity solution.
摘要翻译： 用于分离纯形式的维生素B12的方法，其包括使具有杂质的含维生素B12的液体与二乙烯基苯苯乙烯共聚物树脂接触，并使维生素B12吸附到树脂上。 将这样吸附的维生素B12洗脱洗脱液，洗脱液是含有选自下酮，低级酯和低级醚的化合物的水溶液。 可以用纯化水或低酸度水溶液洗脱之前处理树脂。
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