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    PROCESS FOR THE PRODCUTION OF GLYCERIDES WITH LIPASES
    2.
    发明公开
    PROCESS FOR THE PRODCUTION OF GLYCERIDES WITH LIPASES 有权
    VERFAHREN ZUR HERSTELLUNG VON GLYCERIDEN MITTELS LIPASEN

    公开(公告)号：EP1300470A1

    公开(公告)日：2003-04-09

    申请号：EP01945758.9

    申请日：2001-07-02

    申请人： Nippon Suisan Kaisha, Ltd.

    发明人： IRIMESCU, Roxana ,  FURIHATA, Kiyomi ,  HATA, Kazuhiko ,  YAMANE, Tsuneo

    IPC分类号： C12P7/64

    CPC分类号： C12P7/6445 ,  C12P7/6472

    摘要： The invention provides a process for producing 2-monoglycerides (2-MG) or triglycerides (TG), particularly triglycerides having polyunsaturated fatty acid (PUFA) residues in the sn-2 positions, at an extremely high purity and a high efficiency.
    The 2-MG is produced by conducting alcoholysis of a starting TG with a first 1,3-lipase. Then, the TG is produced by introducing fatty acid residues to the 1- and 3-positions of the produced 2-MG by using a second 1,3-lipase. The fatty acid residue in the 2-posiiton is preferably a residue of DHA, EPA or ARA. The "first lipase" refers to one which is selective for the 1- and 3-positions and can act even on a long-chain fatty acid in alcoholysis, while the "second lipase" refers to one which is selective for the 1- and 3-positions and can produce a TG through the reaction of a 2-MG with a fatty acid ester or a free fatty acid.

    摘要翻译： 本发明提供了以非常高的纯度和高效率生产2-单甘油酯（2-MG）或甘油三酸酯（TG），特别是在sn-2位置具有多不饱和脂肪酸（PUFA）残基的甘油三酯的方法。 通过用第一种1,3-脂肪酶进行起始TG的醇解来制备2-MG。 然后，通过使用第二种1,3-脂肪酶将所产生的2-MG的1-和3-位引入脂肪酸残基来产生TG。 2-位的脂肪酸残基优选为DHA，EPA或ARA的残基。 “第一脂肪酶”是指对于1-和3-位是选择性的，并且在醇解中甚至可以作用于长链脂肪酸，而“第二脂肪酶”是指对于1-和 3-位，并且可以通过2-MG与脂肪酸酯或游离脂肪酸的反应产生TG。