公开(公告)号：EP3936604A1

公开(公告)日：2022-01-12

申请号：EP20765947.5

申请日：2020-01-22

申请人： NISSIN FOODS HOLDINGS CO., LTD. ,  The University of Tokyo

发明人： TAKEUCHI, Shoji ,  MORIMOTO, Yuya ,  SHIMA, Ai ,  FURUHASHI, Mai

IPC分类号： C12M3/00 ,  C12N5/077

摘要： The present invention provides a production method for a three-dimensional muscle tissue including the steps of: preparing an approximately rectangular first cell module containing skeletal myoblasts in a hydrogel, and having a plurality of approximately rectangular holes parallel to each other, and an approximately rectangular second cell module containing skeletal myoblasts in a hydrogel, and having a plurality of approximately rectangular holes parallel to each other at positions different from those of the first cell module in a vertical direction; alternately stacking the prepared first cell module and the prepared second cell module to obtain a stack; subjecting the skeletal myoblasts contained in the obtained stack to proliferation culture; and inducing the proliferated skeletal myoblasts to differentiate into myotubes.