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公开(公告)号：EP0631585B1
公开(公告)日：1998-09-30
申请号：EP93901661.4
申请日：1992-12-11
申请人： NOVO NORDISK A/S
发明人： JOERGENSEN, Per, Linaa , PEDERSEN, Poul, Erik , PETERSEN, Joergen , NIELSEN, Torben, K. , MIKKELSEN, Jan, Moeller
摘要： A process for the microbial production of a protein susceptible to inactivation in a fluid production medium by constinuously and reversibly protecting said protein against said inactivation during the production stage, separating the protein from the production medium, deprotecting the protein, and recovering the protein product. The process is especially useful for obtaining substantially increased yields of the protein in question by continuously precipitating said protein.
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公开(公告)号：EP0631585A1
公开(公告)日：1995-01-04
申请号：EP93901661.0
申请日：1992-12-11
申请人： NOVO NORDISK A/S
发明人： JOERGENSEN, Per, Linaa , PEDERSEN, Poul, Erik , PETERSEN, Joergen , NIELSEN, Torben, K. , MIKKELSEN, Jan, Moeller
摘要： A process for the microbial production of a protein susceptible to inactivation in a fluid production medium by constinuously and reversibly protecting said protein against said inactivation during the production stage, separating the protein from the production medium, deprotecting the protein, and recovering the protein product. The process is especially useful for obtaining substantially increased yields of the protein in question by continuously precipitating said protein.
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公开(公告)号：EP0817856A1
公开(公告)日：1998-01-14
申请号：EP96906704.0
申请日：1996-03-22
申请人： NOVO NORDISK A/S
发明人： TANGNEY, Martin , HANSEN, Christian , PEDERSEN, Poul, Erik , JORGENSEN, Per, Lina , JORGENSEN, Steen, Troels
摘要： A method of constructing recombinant Bacillus strains encoding a polypeptide of interest, in which method a DNA construct comprising a DNA sequence encoding the polypeptide of interest is introduced into a recipient Bacillus cell by conjugation from a donor cell.
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