公开(公告)号：EP1295941A1

公开(公告)日：2003-03-26

申请号：EP01953279.5

申请日：2001-06-27

申请人： National Institute of Advanced Industrial Science and Technology ,  Kankyo Engineering Co., Ltd.

发明人： KURANE, R. Nat. Inst. of Adv. Ind. Scie. & Tech. ,  KANAGAWA, T. Nat. Inst. of Adv. Ind. Scie. & Tech. ,  KAMAGATA, Y. Nat. Inst. of Adv. Ind. Scie. & Tech. ,  TORIMURA, M. Nat. Inst. of Adv. Ind. Scie. & Tech. ,  KURATA, Shinya Kankyo Engineering Co., Ltd. ,  YAMADA, Kazutaka Kankyo Engineering Co., Ltd. ,  YOKOMAKU, Toyokazu Kankyo Engineering Co., Ltd.

IPC分类号： C12N15/09 ,  C12M1/00 ,  C12M1/34 ,  C12Q1/68

CPC分类号： C12Q1/6818 ,  C12Q1/6851 ,  Y02A90/26 ,  C12Q2565/107 ,  C12Q2565/1015

摘要： (1) A novel nucleic acid probe comprising a single-stranded oligonucleotide labeled with a fluorescent dye such that, when the nucleic acid probe is hybridized with a target nucleic acid, the intensity of fluorescence in a hybridization reaction system decreases after a hybridization reaction, and (2) a novel nucleic acid probe comprising a single-stranded oligonucleotide labeled with a fluorescent dye and a quencher substance such that the intensity of fluorescence in a hybridization reaction system increases after a hybridization reaction, the latter oligonucleotide forming no stem-loop structure between bases at positions where the oligonucleotide is labeled with the fluorescent dye and the quencher substance, respectively; various determination kits and DNA chips making use of these probes; method for determining nucleic acids, polymorphous analysis methods and real-time quantitative PCR methods, all of which make use of the determination kits or DNA chips.