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1.发明授权NOVEL MIXTURES FOR ASSAYING NUCLEIC ACID, NOVEL METHOD OF ASSAYING NUCLEIC ACID WITH THE USE OF THE SAME AND NUCLEIC ACID PROBE TO BE USED THEREFOR 有权用于测试NUCLEIC，新方法对于核酸测试使用其确保新的产品配剂;适用NUCLEIC PROBE
公开(公告)号：EP1698897B1
公开(公告)日：2010-03-17
申请号：EP04807404.1
申请日：2004-12-20
申请人： Nippon Steel Kankyo Engineering Co., Ltd. , National Institute of Advanced Industrial Science and Technology
发明人： NAKAMURA, Kazunori, Nat.Inst.Advanced Ind.Sci&Tech , KANAGAWA, Takahiro, Nat.Inst.Advanced Ind.Sci&Tech , NODA, Naohiro, Nat.Inst.Advanced Industr.Sci&Tech , TSUNEDA, Satoshi, c/o Waseda University , TANI, Hidenori, Waseda University , KURATA, Shinya, c/o Kankyo Engineering Co.,Ltd.
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6818 , Y10T436/143333 , C12Q2545/107
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公开(公告)号：EP1550717A1
公开(公告)日：2005-07-06
申请号：EP03733227.7
申请日：2003-05-30
申请人： Kankyo Engineering Co., Ltd. , National Institute of Advanced Industrial Science and Technology
发明人： KURATA, Shinya, KANKYO ENGINEERING CO., LTD. , TAKATSU, Kyoko, KANKYO ENGINEERING CO., LTD. , NAKAMURA, Kazunori, Nat.Inst.Adv.Ind.Science&Techn , KANAGAWA, Takahiro, Nat.Inst.Adv.Ind.Science&Techn
CPC分类号： C12Q1/6844 , C12Q2563/107
摘要： A novel method is provided to assay at least one nucleic acid. According to this method, a nucleic acid polymerization reaction is conducted in a nucleic acid polymerization reaction system, which contains (A) the at least one nucleic acid as a template, (B) at least one nucleotide monomer labeled with at least one label selected from the group consisting of (a) fluorescent dyes, (b) quenchers and (c) immune related substances with a fluorescent dye or quencher contained therein, and (C) at least one nucleic acid-synthesizing enzyme . The template nucleic acid or a nucleic acid, which has been synthesized using the template nucleic acid as a template, is then assayed from a change or an amount of a change in an optical character of the nucleic acid polymerization system. This method makes it possible to specifically and accurately assay at least one nucleic acid, which is contained in a single system and can be an unknown nucleic acid and/or a known nucleic acid, with excellent sensitivity, in short time and with ease.
摘要翻译： 本发明提供一种新颖的方法来测定至少一种核酸。 。根据该方法，一个核酸聚合反应是在所选择的核酸聚合反应系统，它含有（A）所述至少一种核酸为模板，（B）至少一个核苷酸单体用至少一个标签传导 选自（a）中的荧光染料，（b）和淬灭剂用荧光染料（c）中的免疫相关物质或猝灭剂包含在其中，和（C）至少一种核酸合成酶。 模板核酸或核酸，已经使用所述模板核酸为模板合成，然后从一个变化测定或达在核酸聚合体系的光学字符中的变化的，该方法使得有可能 特异性和精确地设定测定法，其被包含在一个单一的系统，并且可以是未知的核酸和/或公知的核酸的，具有优良的灵敏度，在很短的时间且容易的至少一种核酸，全部。
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3.发明公开NOVEL NUCLEIC ACID PROBES AND METHOD OF ASSAYING NUCLEIC ACID BY USING THE SAME 有权用于核酸测试新的核酸探针和方法使用THEREOF
公开(公告)号：EP1295941A4
公开(公告)日：2004-06-16
申请号：EP01953279
申请日：2001-06-27
发明人： KANAGAWA TAKAHIRO , KURANE RYUICHIRO , YAMADA KAZUTAKA , YOKOMAKU TOYOKAZU , TORIMURA MASAKI , KAMAGATA YOICHI , KURATA SHINYA
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6818 , C12Q1/6851 , Y02A90/26 , C12Q2565/107 , C12Q2565/1015
摘要： Nucleic acid probes are provided, each of which is formed of a single-stranded oligonucleotide which can hybridize to a target nucleic acid and is labeled with a fluorescent dye or with a fluorescent dye and a quencher substance. The nucleic acid probes can be easily designed, permit determination, polymorphous analysis or real-time quantitative PCR of nucleic acids in short time, and are not dissociated during reactions. Nucleic acid determination methods, polymorphous analysis methods and real-time quantitative PCR methods, which make use of the nucleic acid probes, are also provided.
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4.发明公开NOVEL NUCLEIC ACID PROBE AND NOVEL METHOD OF ASSAYING NUCLEIC ACIDS USING THE SAME 审中-公开新的核酸探针和核酸分析使用相同的新方法
公开(公告)号：EP1321520A4
公开(公告)日：2003-09-03
申请号：EP02713215
申请日：2002-03-27
发明人： KURANE RYUICHIRO , KANAGAWA TAKAHIRO , KAMAGATA YOICHI , TORIMURA MASAKI , KURATA SHINYA , YAMADA KAZUTAKA , YOKOMAKU TOYOKAZU
IPC分类号： C12Q1/68 , G01N33/58 , C12N15/09 , G01N33/53 , G01N33/566
CPC分类号： G01N33/582 , C12Q1/6818 , C12Q2565/101 , C12Q2565/107 , C12Q2565/1015
摘要： A novel nucleic acid probe for simultaneously assaying nucleic acids with a simple apparatus in case where one or more nucleic acids are present in the assay system, characterized in that, in a single-stranded nucleic acid probe labeled with a plural number of fluorescent pigments so as to form at least one pair of fluorescent pigments causing the FRET phenomenon (i.e., a fluorescent pigment capable of serving as a donor pigment and another fluorescent pigment capable of serving as an acceptor pigment), the base sequence is designed so that the fluorescent intensity of the acceptor pigment is weakened when the probe is hybridized with the target nucleic acids, and the above pigments are labeled.
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公开(公告)号：EP2319922B1
公开(公告)日：2015-10-14
申请号：EP08791869.4
申请日：2008-07-30
CPC分类号： C12Q1/6818 , C12Q1/6827 , C12Q1/6858 , C12Q2537/125 , C12Q2525/161 , C12Q2565/107 , C12Q2525/191 , C12Q2565/101 , C12Q2525/155
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6.发明授权
公开(公告)号：EP1046717B1
公开(公告)日：2010-10-06
申请号：EP00108643.8
申请日：2000-04-20
申请人： National Institute of Advanced Industrial Science and Technology , Nippon Steel Kankyo Engineering Co., Ltd.
发明人： Kurane, Ryuichiro, Nat.Inst.of Biosci&Human-Techn. , Kanagawa, Takahiro Nat.Inst.of Biosci&Human-Techn. , Kamagata, Yoichi, Nat.Inst.of Biosci&Human-Techn. , Kurata, Shinya, c/o Kankyo Engineering Co.,Ltd. , Yamada, Kazutaka, c/o Kankyo Engineering Co.,Ltd. , Yokomaku, Toyokazu, c/o Kankyo Engineering Co.,Ltd , Koyama, Osamu, c/o Kankyo Engineering Co.,Ltd. , Furusho, Kenta, c/o Kankyo Engineering Co. Ltd.,
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6816 , C12Q1/6827 , C12Q1/6837 , C12Q1/6851 , C12Q1/6858 , C12Q2565/549 , C12Q2565/107 , C12Q2563/107 , C12Q2525/185
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7.发明公开NOVEL MIXTURES FOR ASSAYING NUCLEIC ACID, NOVEL METHOD OF ASSAYING NUCLEIC ACID WITH THE USE OF THE SAME AND NUCLEIC ACID PROBE TO BE USED THEREFOR 有权用于测试NUCLEIC，新方法对于核酸测试使用其确保新的产品配剂;适用NUCLEIC PROBE
公开(公告)号：EP1698897A1
公开(公告)日：2006-09-06
申请号：EP04807404.1
申请日：2004-12-20
申请人： Kankyo Engineering Co., Ltd. , National Institute of Advanced Industrial Science and Technology
发明人： NAKAMURA, Kazunori, Nat.Inst.Advanced Ind.Sci&Tech , KANAGAWA, Takahiro, Nat.Inst.Advanced Ind.Sci&Tech , NODA, Naohiro, Nat.Inst.Advanced Industr.Sci&Tech , TSUNEDA, Satoshi, c/o Waseda University , TANI, Hidenori, Waseda University , KURATA, Shinya, c/o Kankyo Engineering Co.,Ltd.
CPC分类号： C12Q1/6818 , Y10T436/143333 , C12Q2545/107
摘要： [Problems] To provide a novel mixture for assaying a target nucleic acid, characterized by enabling a nucleic acid assay while: 1) requiring no step of diluting the target nucleic acid; 2) requiring no procedure of changing a probe concentration depending on a concentration of the target nucleic acid.
[Means for Solving Problems] 1) A mixture which comprises one internal standard nucleic acid and two nucleic acid probes labeled with a fluorescent dye; 2) a mixture for measuring Km value which comprises one internal standard nucleic acid having a partial mutation and one nucleic acid probe labeled with a fluorescent dye; 3)a mixture which comprises one internal standard nucleic acid and one double nucleic acid probe labeled with two fluorescent dyes; and a method for assaying a nucleic acid by making use thereof.摘要翻译： [问题]为了用于测定靶核酸，通过使核酸测定法为特征提供一种新颖的混合物中，同时：1）无需任何稀释靶核酸的步骤; 2）没有需要更换的样品浓度根据目标核酸的浓度的步骤。 [用于解决问题的方案1），它包括一个内标核酸和标记有荧光染料两个核酸探针的混合物; 2），用于测量的Km值，其包括具有一个局部突变和标记有荧光染料一种核酸探针一个内标核酸的混合物; 3）包括一个内标核酸和标记有两种荧光染料一个双键的核酸探针的混合物; 及用于利用其测定的核酸的方法。
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公开(公告)号：EP1046717A3
公开(公告)日：2004-01-21
申请号：EP00108643.8
申请日：2000-04-20
申请人： National Institute of Advanced Industrial Science and Technology , Kankyo Engineering Co., Ltd.
发明人： Kurane, Ryuichiro, Nat.Inst.of Biosci&Human-Techn. , Kanagawa, Takahiro Nat.Inst.of Biosci&Human-Techn. , Kamagata, Yoichi, Nat.Inst.of Biosci&Human-Techn. , Kurata, Shinya, c/o Kankyo Engineering Co.,Ltd. , Yamada, Kazutaka, c/o Kankyo Engineering Co.,Ltd. , Yokomaku, Toyokazu, c/o Kankyo Engineering Co.,Ltd , Koyama, Osamu, c/o Kankyo Engineering Co.,Ltd. , Furusho, Kenta, c/o Kankyo Engineering Co. Ltd.,
IPC分类号： C12Q1/68
摘要： A method is provided for determining a concentration of a target nucleic acid by using a nucleic acid probe labeled with a fluorescent dye. The method comprises: providing, as the probe, a nucleic acid probe capable of reducing fluorescence emission from the fluorescent dye when hybridized with the target nucleic acid; hybridizing the probe to the target nucleic acid; and measuring a decrease in fluorescence emission from the fluorescent dye after the hybridization relative to fluorescence emission from the fluorescent dye before the hybridization.
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9.发明公开NOVEL NUCLEIC ACID PROBES AND METHOD OF ASSAYING NUCLEIC ACID BY USING THE SAME 有权新的是核酸探针和方法对于核酸其用途正在测试
公开(公告)号：EP1295941A1
公开(公告)日：2003-03-26
申请号：EP01953279.5
申请日：2001-06-27
申请人： National Institute of Advanced Industrial Science and Technology , Kankyo Engineering Co., Ltd.
发明人： KURANE, R. Nat. Inst. of Adv. Ind. Scie. & Tech. , KANAGAWA, T. Nat. Inst. of Adv. Ind. Scie. & Tech. , KAMAGATA, Y. Nat. Inst. of Adv. Ind. Scie. & Tech. , TORIMURA, M. Nat. Inst. of Adv. Ind. Scie. & Tech. , KURATA, Shinya Kankyo Engineering Co., Ltd. , YAMADA, Kazutaka Kankyo Engineering Co., Ltd. , YOKOMAKU, Toyokazu Kankyo Engineering Co., Ltd.
CPC分类号： C12Q1/6818 , C12Q1/6851 , Y02A90/26 , C12Q2565/107 , C12Q2565/1015
摘要： (1) A novel nucleic acid probe comprising a single-stranded oligonucleotide labeled with a fluorescent dye such that, when the nucleic acid probe is hybridized with a target nucleic acid, the intensity of fluorescence in a hybridization reaction system decreases after a hybridization reaction, and (2) a novel nucleic acid probe comprising a single-stranded oligonucleotide labeled with a fluorescent dye and a quencher substance such that the intensity of fluorescence in a hybridization reaction system increases after a hybridization reaction, the latter oligonucleotide forming no stem-loop structure between bases at positions where the oligonucleotide is labeled with the fluorescent dye and the quencher substance, respectively; various determination kits and DNA chips making use of these probes; method for determining nucleic acids, polymorphous analysis methods and real-time quantitative PCR methods, all of which make use of the determination kits or DNA chips.
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10.发明公开
公开(公告)号：EP1046717A9
公开(公告)日：2001-06-27
申请号：EP00108643
申请日：2000-04-20
发明人： KURANE RYUICHIRO , KANAGAWA TAKAHIRO , KAMAGATA YOICHI , KURATA SHINYA , YAMADA KAZUTAKA , YOKOMAKU TOYOKAZU , KOYAMA OSAMU , FURUSHO KENTA
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6816 , C12Q1/6827 , C12Q1/6837 , C12Q1/6851 , C12Q1/6858 , C12Q2565/549 , C12Q2565/107 , C12Q2563/107 , C12Q2525/185
摘要： A method is provided for determining a concentration of a target nucleic acid by using a nucleic acid probe labeled with a fluorescent dye. The method comprises:
providing, as the probe, a nucleic acid probe capable of reducing fluorescence emission from the fluorescent dye when hybridized with the target nucleic acid;
hybridizing the probe to the target nucleic acid; and measuring a decrease in fluorescence emission from the fluorescent dye after the hybridization relative to fluorescence emission from the fluorescent dye before the hybridization.
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