专利检索 ap:("National Institute of Advanced Industrial Science AND Technology" OR "Kankyo Engineering Co., Ltd.") AND inv:"Kanagawa, Takahiro Nat.Inst.of Biosci&Human-Techn." 第 1 页

1.

发明公开
Method and probes for determining a concentration of target nucleic acid molecules and method for analyzing data obtained by the method 审中-公开

公开(公告)号：EP1046717A3

公开(公告)日：2004-01-21

申请号：EP00108643.8

申请日：2000-04-20

申请人： National Institute of Advanced Industrial Science and Technology , Kankyo Engineering Co., Ltd.

发明人： Kurane, Ryuichiro, Nat.Inst.of Biosci&Human-Techn. , Kanagawa, Takahiro Nat.Inst.of Biosci&Human-Techn. , Kamagata, Yoichi, Nat.Inst.of Biosci&Human-Techn. , Kurata, Shinya, c/o Kankyo Engineering Co.,Ltd. , Yamada, Kazutaka, c/o Kankyo Engineering Co.,Ltd. , Yokomaku, Toyokazu, c/o Kankyo Engineering Co.,Ltd , Koyama, Osamu, c/o Kankyo Engineering Co.,Ltd. , Furusho, Kenta, c/o Kankyo Engineering Co. Ltd.,

IPC分类号： C12Q1/68

摘要： A method is provided for determining a concentration of a target nucleic acid by using a nucleic acid probe labeled with a fluorescent dye. The method comprises: providing, as the probe, a nucleic acid probe capable of reducing fluorescence emission from the fluorescent dye when hybridized with the target nucleic acid; hybridizing the probe to the target nucleic acid; and measuring a decrease in fluorescence emission from the fluorescent dye after the hybridization relative to fluorescence emission from the fluorescent dye before the hybridization.