公开(公告)号：EP3912992A1

公开(公告)日：2021-11-24

申请号：EP20752298.8

申请日：2020-02-04

申请人： National University Corporation Ehime University

发明人： OCHI Toshiki ,  YASUKAWA Masaki ,  FUJIWARA Hiroshi ,  TAKENAKA Katsuto

IPC分类号： C07K14/47 ,  C07K14/705 ,  C07K16/00 ,  C07K16/46 ,  C07K19/00 ,  C40B40/08 ,  C40B40/10 ,  C12N5/0783 ,  C12N5/10 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N15/13 ,  C12N15/62 ,  C12Q1/02 ,  C12Q1/04 ,  C40B30/04 ,  C40B50/06 ,  G01N33/531 ,  C12P21/02 ,  C12P21/08

摘要： Provided are a CAR library used to screen scFvs that can be functional in CAR-T cells, and an scFv manufacturing method in which the CAR library is used. A chimeric antigen receptor (CAR) library of the present invention includes nucleic acids coding for first CARs. Each of the first CARs includes a first antigen-binding domain, a first transmembrane domain, and a first intracellular signaling domain. The first antigen-binding domain includes a first single-chain antibody (scFv) to be screened for the ability to bind to a target antigen. The first scFv includes a first heavy-chain variable region and a first light-chain variable region. The first heavy-chain variable region and the first light-chain variable region meet a predetermined condition.