公开(公告)号：EP4130278A1

公开(公告)日：2023-02-08

申请号：EP21773444.1

申请日：2021-03-26

申请人： National University Corporation Kagawa University

发明人： AKIMITSU, Kazuya ,  IZUMORI, Ken ,  YOSHIHARA, Akihide ,  KATO, Shiro ,  MOCHIZUKI, Susumu ,  YOSHIDA, Hiromi ,  KAMITORI, Shigehiro

IPC分类号： C12N15/61 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N9/90 ,  C12N11/082 ,  C12N15/63 ,  C12P19/24

摘要： A highly safe, highly active, and heat-resistant L-rhamnose isomerase and an amino acid-substituted mutant thereof, a microorganism producing them, or a method for producing an aldose or a ketose are provided, all of which are capable of being used in the food industry.
L-Rhamnose isomerase, which is capable of being obtained from a microorganism belonging to the genus Erwinia, has a 48 kDa subunit molecular mass measured by SDS-PAGE, and has the following substrate specificities (A) and (B).
(A) An isomerase activity of recognizing and reacting with a CHO group of C1 and an OH group of C2 of an aldose to convert the CHO group of C1 to an OH group and the OH group of C2 to a CO group, alternatively, recognizing and reacting with an OH group of C1 and a CO group of C2 of a ketose to convert the OH group of C1 to a CHO group and the CO group of C2 to an OH group.
(B) An activity of catalyzing an isomerization reaction between L-rhamnose and L-rhamnulose, between L-lyxose and L-xylulose, between L-mannose and L-fructose, between D-ribose and D-ribulose, between L-talose and L-tagatose, and between D-allose and D-allulose.

公开(公告)号：EP4257687A1

公开(公告)日：2023-10-11

申请号：EP21898178.5

申请日：2021-11-30

申请人： National University Corporation Kagawa University

发明人： AKIMITSU, Kazuya ,  IZUMORI, Ken ,  YOSHIHARA, Akihide ,  KATO, Shiro ,  MOCHIZUKI, Susumu ,  YOSHIDA, Hiromi ,  KAMITORI, Shigehiro

IPC分类号： C12N15/61 ,  C12N1/15 ,  C12N1/19 ,  C12N1/20 ,  C12N1/21 ,  C12N5/10 ,  C12N9/90 ,  C12N11/00 ,  C12N11/08 ,  C12N15/31 ,  C12N15/63 ,  C12P19/02

摘要： Problem: to provide a highly safe and highly heat-resistant L-rhamnose isomerase usable in the food industry, a microorganism for producing the same, and a method of producing an aldose or ketose.
Solution: an L-rhamnose isomerase derived from a microorganism belonging to the genus Enterobacter, having a subunit molecular mass of 47 kDa as measured by SDS-PAGE, and having the following substrate specificities (A) and (B).
(A) having isomerase activity that recognizes and reacts with a CHO group at C1 and an OH group at C2 of an aldose to convert the CHO group at C1 into an OH group and the OH group at C2 into a CO group, or recognizes and reacts with an OH group at C1 and a CO group at C2 of a ketose to convert the OH group at C1 into a CHO group and the CO group at C2 into an OH group.
(B) having reactivity with L-rhamnose, L-lyxose, L-mannose, D-allose, D-ribose, and L-talose.

公开(公告)号：EP3885442A1

公开(公告)日：2021-09-29

申请号：EP19887837.3

申请日：2019-11-21

申请人： National University Corporation Kagawa University

发明人： AKIMITSU, Kazuya ,  IZUMORI, Ken ,  YOSHIHARA, Akihide ,  KATO, Shiro ,  MOCHIZUKI, Susumu ,  YOSHIDA, Hiromi ,  KAMITORI, Shigehiro

IPC分类号： C12P19/02 ,  C12N5/10 ,  C12N1/15 ,  C12N1/19 ,  C12N1/20 ,  C12N1/21 ,  C12N15/61 ,  C12N15/63 ,  C12N9/90 ,  C12N11/02

摘要： To provide a ketose 3-epimerase that can be used in the food industry and is highly safe, highly active, and heat resistant, a microorganism that produces this enzyme, and a method of producing a ketose. The present invention provides a ketose 3-epimerase that can be obtained from a microorganism belonging to the genus Arthrobacter , has a molecular mass of about 36 kDa as measured by SDS-PAGE, and has the following substrate specificities (A) and (B):
(A) having an activity of epimerizing position 3 of a D- or L-ketose and preparing a D- or L-ketose corresponding thereto, and
(B) having the highest epimerization activity on position 3 of D-allulose, among D- or L-ketohexoses.