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公开(公告)号:EP0460217A1
公开(公告)日:1991-12-11
申请号:EP90917547.3
申请日:1990-11-28
申请人: Nippon Oil Co., Ltd.
CPC分类号: C07K14/005 , C12N15/11 , C12N15/8203 , C12N2770/00022
摘要: A DNA sequence corresponding to the gene of Odontoglossum ring spot virus (ORSV) is prepared by gene recombination technique and used to express a virus protein with a host cell. This sequence is particularly useful as a material for developing a vector for plant gene recombination.
摘要翻译: cDNA序列对应。 (ORSV)的基因组和具有特定结构的双链DNA(每条链上5977bp)。 通过开环cDNA,是新的。 还要求的是基因载体contg。 上述DNA和转化体生物(例如大肠杆菌,枯草芽孢杆菌,农杆菌或植物细胞) 矢量。 转化体的培养物产生一种mol.wt. 33,000对应。 对病毒外壳蛋白。 县。 双链DNA是可以接受的。 从EcoRI切割cDNA。
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公开(公告)号:EP0460217B1
公开(公告)日:1994-11-09
申请号:EP90917547.3
申请日:1990-11-28
申请人: Nippon Oil Co., Ltd.
CPC分类号: C07K14/005 , C12N15/11 , C12N15/8203 , C12N2770/00022
摘要: A DNA sequence corresponding to the gene of Odontoglossum ring spot virus (ORSV) is prepared by gene recombination technique and used to express a virus protein with a host cell. This sequence is particularly useful as a material for developing a vector for plant gene recombination.
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