公开(公告)号：EP1550717B1

公开(公告)日：2010-09-08

申请号：EP03733227.7

申请日：2003-05-30

申请人： Nippon Steel Kankyo Engineering Co., Ltd. ,  National Institute of Advanced Industrial Science and Technology

发明人： KURATA, Shinya, KANKYO ENGINEERING CO., LTD. ,  TAKATSU, Kyoko, KANKYO ENGINEERING CO., LTD. ,  NAKAMURA, Kazunori, Nat.Inst.Adv.Ind.Science&Techn ,  KANAGAWA, Takahiro, Nat.Inst.Adv.Ind.Science&Techn

IPC分类号： C12N15/00 ,  C12Q1/68

CPC分类号： C12Q1/6844 ,  C12Q2563/107

摘要： It is possible to provide a novel method whereby at least one nucleic acid such as an unknown nucleic acid and/or a known nucleic acid contained in a system can be conveniently, specifically and accurately assayed at a high sensitivity within a short period of time. Namely, a method of assaying a nucleic acid characterized by comprising effecting a nucleic acid polymerization reaction in a nucleic acid polymerization system which contains (A) at least one nucleic acid as a template, (B) at least one nucleotide monomer labeled with at least one substance selected from among (a) a fluorescent dye, (b) a quencher and (c) an immune-associated substance containing a fluorescent dye or a quencher, and (C) at least one nucleic acid synthase, and assaying the template nucleic acid or a nucleic acid synthesized by using the same as a template based on a change in the optical characters of the nucleic acid polymerization system or a quantitative change thereof.

公开(公告)号：EP1550717A1

公开(公告)日：2005-07-06

申请号：EP03733227.7

申请日：2003-05-30

申请人： Kankyo Engineering Co., Ltd. ,  National Institute of Advanced Industrial Science and Technology

发明人： KURATA, Shinya, KANKYO ENGINEERING CO., LTD. ,  TAKATSU, Kyoko, KANKYO ENGINEERING CO., LTD. ,  NAKAMURA, Kazunori, Nat.Inst.Adv.Ind.Science&Techn ,  KANAGAWA, Takahiro, Nat.Inst.Adv.Ind.Science&Techn

IPC分类号： C12N15/00 ,  C12Q1/68

CPC分类号： C12Q1/6844 ,  C12Q2563/107

摘要： A novel method is provided to assay at least one nucleic acid. According to this method, a nucleic acid polymerization reaction is conducted in a nucleic acid polymerization reaction system, which contains (A) the at least one nucleic acid as a template, (B) at least one nucleotide monomer labeled with at least one label selected from the group consisting of (a) fluorescent dyes, (b) quenchers and (c) immune related substances with a fluorescent dye or quencher contained therein, and (C) at least one nucleic acid-synthesizing enzyme . The template nucleic acid or a nucleic acid, which has been synthesized using the template nucleic acid as a template, is then assayed from a change or an amount of a change in an optical character of the nucleic acid polymerization system. This method makes it possible to specifically and accurately assay at least one nucleic acid, which is contained in a single system and can be an unknown nucleic acid and/or a known nucleic acid, with excellent sensitivity, in short time and with ease.

摘要翻译： 本发明提供一种新颖的方法来测定至少一种核酸。 。根据该方法，一个核酸聚合反应是在所选择的核酸聚合反应系统，它含有（A）所述至少一种核酸为模板，（B）至少一个核苷酸单体用至少一个标签传导 选自（a）中的荧光染料，（b）和淬灭剂用荧光染料（c）中的免疫相关物质或猝灭剂包含在其中，和（C）至少一种核酸合成酶。 模板核酸或核酸，已经使用所述模板核酸为模板合成，然后从一个变化测定或达在核酸聚合体系的光学字符中的变化的，该方法使得有可能 特异性和精确地设定测定法，其被包含在一个单一的系统，并且可以是未知的核酸和/或公知的核酸的，具有优良的灵敏度，在很短的时间且容易的至少一种核酸，全部。