公开(公告)号：EP4067485A3

公开(公告)日：2023-01-04

申请号：EP22154905.8

申请日：2015-12-01

Applicant: Novozymes A/S

Inventor： SVENDSEN, Allan ,  VIND, Jesper ,  BAUNSGAARD, Lone ,  SKJOLD-JØRGENSEN, Jakob ,  BHATIA, Vikram, Kjøller ,  OESTERGAARD, Lars, Henrik,

IPC: C12N9/18 ,  C12N9/20

Abstract: The present invention relates to a variant of a parent lipase, wherein said variant has at least 60% but less than 100% sequence identity with SEQ ID NO: 2, has lipase activity, comprises a substitution at a position corresponding to position 92 and/or 96; and remains unaltered at the positions corresponding to positions 231, 233 and 254 of SEQ ID NO: 2. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.

公开(公告)号：EP4067485A2

公开(公告)日：2022-10-05

申请号：EP22154905.8

申请日：2015-12-01

Applicant: Novozymes A/S

Inventor： SVENDSEN, Allan ,  VIND, Jesper ,  BAUNSGAARD, Lone ,  SKJOLD-JØRGENSEN, Jakob ,  BHATIA, Vikram, Kjøller ,  OESTERGAARD, Lars, Henrik,

IPC: C12N9/18 ,  C12N9/20

Abstract: The present invention relates to a variant of a parent lipase, wherein said variant has at least 60% but less than 100% sequence identity with SEQ ID NO: 2, has lipase activity, comprises a substitution at a position corresponding to position 92 and/or 96; and remains unaltered at the positions corresponding to positions 231, 233 and 254 of SEQ ID NO: 2. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.

公开(公告)号：EP3878957A1

公开(公告)日：2021-09-15

申请号：EP21156489.3

申请日：2015-05-26

Applicant: Novozymes A/S

Inventor： SVENDSEN, Allan ,  SAIKIA, Rakhi ,  DALAL, Sohel ,  BHATIA, Vikram, Kjøller ,  BAUNSGAARD, Lone

IPC: C12N9/20 ,  C12N15/52 ,  C12N1/21 ,  C12N1/15

Abstract: The invention relates to a method for generating a preparation of lipase with increased lipolytic activity comprising a step of altering one or more nucleotides in a polynucleotide comprising a first polynucleotide encoding a propeptide operationally linked to a second polynucleotide encoding a lipase, wherein the alteration of one or more nucleotides are made in the first polynucleotide and the alteration independently results in a substitution, an insertion or a deletion in the encoded propeptide amino acid sequence