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1.发明公开ENZYME VARIANTS AND POLYNUCLEOTIDES ENCODING SAME 审中-公开ENZYMVARIANTEN UND POLYNUKLEOTIDE ZUR CODIERUNG DAVON
公开(公告)号：EP3122762A1
公开(公告)日：2017-02-01
申请号：EP15712656.6
申请日：2015-03-30
申请人： Novozymes A/S
CPC分类号： C12N9/54 , C11D3/386 , C11D3/38681 , C12Y304/00
摘要： The present invention relates to enzyme variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; methods of using the variants and composition comprising variants.
摘要翻译： 本发明涉及酶变体。 本发明还涉及编码这些变体的多核苷酸; 核酸构建体，载体和包含多核苷酸的宿主细胞; 使用包含变体的变体和组合物的方法。
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2.发明公开RESOLUBILIZATION OF PROTEIN CRYSTALS AT LOW PH 审中-公开RESOLUBILISIERUNG VON PROTEINKRISTALLEN BEI NIEDRIGEM PH-WERT
公开(公告)号：EP3122760A1
公开(公告)日：2017-02-01
申请号：EP15713724.1
申请日：2015-03-30
申请人： Novozymes A/S
CPC分类号： C07K1/145 , C07K2319/21 , C12N9/2462 , C12N9/54 , C12P21/02 , C12Y302/01017 , C12Y304/21062
摘要： A method for purifying a protein product prepared in a fermentation process where the protein of interest is present in solid, crystalline or amorphous form in the fermentation container is disclosed, where the pH of the fermentation broth is adjusted to a low pH whereby the protein of interest dissolves and can be efficiently separated from insoluble.
摘要翻译： 公开了一种纯化在发酵过程中制备的蛋白质产物的方法，其中所述目标蛋白以固体，结晶或无定形形式存在于发酵容器中，其中发酵液的pH被调节至低pH， 兴趣溶解并且可以有效地从不溶性分离。
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公开(公告)号：EP2742145A1
公开(公告)日：2014-06-18
申请号：EP12745476.7
申请日：2012-08-03
申请人： Novozymes A/S
发明人： PERSSON, Jon Martin , BANKE, Niels
IPC分类号： C12P21/00
CPC分类号： C12N9/2417 , C12P21/00
摘要： A method of producing an enzyme of interest in a fed-batch cultivation comprising: a) cultivating a microorganism in a culture medium conducive to its growth wherein the microorganism produces the enzyme of interest; and b) adding a manganese compound to the culture medium one or more times during the cultivation.
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