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    USE OF A PHENOL OXIDISING ENZYME IN THE TREATMENT OF TOBACCO
    2.
    发明公开
    USE OF A PHENOL OXIDISING ENZYME IN THE TREATMENT OF TOBACCO 有权
    USING A酚酶治疗烟草

    公开(公告)号：EP1094724A1

    公开(公告)日：2001-05-02

    申请号：EP99931028.7

    申请日：1999-07-07

    申请人： Novozymes A/S ,  Imperial Tobacco Limited

    发明人： KIERULFF, Jesper, Vallentin ,  JOERGENSEN, Ole, Bill ,  HANSEN, Tomas, Tage ,  KNOX, Anthony, James ,  DE GRANDPRE, Yves

    IPC分类号： A24B15/24 ,  A24B15/20

    CPC分类号： A24B15/20 ,  A24B15/24

    摘要： A process for preparing tobacco, which process comprises the steps of treating a tobacco material with a phenol oxidising enzyme, such as by extracting tobacco with a solvent to provide an extract and a residue; and treating the extract with a phenol oxidising enzyme such as a laccase. An improved tobacco product having a reduced amount of phenolic compounds. This is an alternative or a supplement to a process in which the phenolic compounds are adsorbed onto the insoluble carrier polyvinylpolypyrrolidone (PVPP). In preferred embodiments, the process includes further steps of removing the oxidised phenolic compound, adding adsorbents such as bentonite; removing and/or inactivating the enzyme; and concentrating the extract. Preferred phenol oxidising enzymes are peroxidases and laccases. The thus treated extract is advantageously re-combined with the tobacco residue and further processed to provide a tobacco article for smoking.