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1.发明公开COMPOSITIONS AND METHODS FOR DIRECTIONAL NUCLEIC ACID AMPLIFICATION AND SEQUENCING 有权组合物和方法用于定向扩增和核酸序列分析
公开(公告)号：EP2769007A1
公开(公告)日：2014-08-27
申请号：EP12842163.3
申请日：2012-10-19
发明人： ARMOUR, Chris
IPC分类号： C40B50/06
CPC分类号： C12N15/1096 , C12N15/1017 , C12N15/1093 , C12N15/66 , C12Q1/6806 , C12Q1/6848 , C12Q1/6869 , C12Q1/6874 , C12Q2521/531 , C12Q2525/191 , C12Q2535/122 , C12Q2537/159
摘要： The invention provides methods and compositions for directional nucleic acid amplification and sequencing. The invention further provides methods and compositions for the construction of directional eDNA libraries. The method may comprise: a) reverse transcribing a RNA sample to generate a first strand eDNA; b) generating a second strand eDNA from the rrrst strand eDNA, wherein at least one of the four dNTPs dATP, dCTP, dGTP or dTTP is replaced by a modified dNTP during second strand synthesis and incorporated into the second strand, thereby generating a double-stranded eDNA; c) performing end repair on the double-stranded eDNA; d) Iigating adaptors to the double-stranded eDIMA, wherein only one of the adaptors has the modified dNTP incorporated into a ligation strand of the adaptor; e) performing gap repair; and f) selectively cleaving the second strand and the ligation strand of the adaptor that has the modified dNTP by a suitable cleavage agent.
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2.发明授权COMPOSITIONS AND METHODS FOR DIRECTIONAL NUCLEIC ACID AMPLIFICATION AND SEQUENCING 有权组合物和方法用于定向扩增和核酸序列分析
公开(公告)号：EP2769007B1
公开(公告)日：2016-12-07
申请号：EP12842163.3
申请日：2012-10-19
发明人： ARMOUR, Chris
IPC分类号： C12N15/10
CPC分类号： C12N15/1096 , C12N15/1017 , C12N15/1093 , C12N15/66 , C12Q1/6806 , C12Q1/6848 , C12Q1/6869 , C12Q1/6874 , C12Q2521/531 , C12Q2525/191 , C12Q2535/122 , C12Q2537/159
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3.发明公开COMPOSITIONS AND METHODS FOR TARGETED NUCLEIC ACID SEQUENCE ENRICHMENT AND HIGH EFFICIENCY LIBRARY GENERATION 审中-公开组合物和有针对性的核序列富集方法及节能图书馆的生产
公开(公告)号：EP2807292A1
公开(公告)日：2014-12-03
申请号：EP13740653.4
申请日：2013-01-25
发明人： AMORESE, Doug , ARMOUR, Chris , KURN, Nurith
IPC分类号： C40B50/06
CPC分类号： C12N15/1068 , C12N15/1072 , C12N15/66 , C12Q2525/191
摘要： The present invention provides methods, compositions and kits for targeted nucleic acid sequence enrichment in a nucleic acid sample and for high efficiency nucleic acid library generation for next generation sequencing (NGS). Specifically, the methods, compositions and kits provided herein are useful for the production and capture of amplification-ready, target-specific and strand-specific regions of interest from nucleic acid samples containing complex DNA.
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