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    Cloning and expression of transforming growth factor beta 2
    1.
    发明公开
    Cloning and expression of transforming growth factor beta 2 失效
    克罗尼昂和表达des transformierenden Wachstumsfaktors beta 2。

    公开(公告)号：EP0376785A2

    公开(公告)日：1990-07-04

    申请号：EP89403480.0

    申请日：1989-12-14

    申请人： ONCOGEN LIMITED PARTNERSHIP

    发明人： Purchio, Anthony F. ,  Madisen, Linda ,  Webb, Nancy

    IPC分类号： C12N15/16 ,  C12N15/85 ,  C12N5/10 ,  C07K13/00 ,  A61K37/02

    CPC分类号： C07K14/495 ,  A61K38/00 ,  C07K2319/00

    摘要： cDNA clones coding for TGF-S2 which are used to construct expression vectors capable of directing the high-level expression of mature, biologically active TGF-β2, as well as precursor TGF-β2 forms, in transfected Chinese Hamster Ovary cells (CHO cells) and transfeced COS cells are described. CHO and COS transfectants secreting TGF-β2 at high levels are also described. CHO cells transfected with a plasmid vector carrying the complete 414 amino acid simian TGF-β2 precursor secrete approximately 5 µg per ml culture media.

    摘要翻译： 编码TGF-β2的cDNA克隆用于构建能够导入成熟的，生物活性的TGF-β2的高水平表达的表达载体以及前体TGF-β2形式的转染的中国仓鼠卵巢细胞（ CHO细胞）和转染的COS细胞。 还描述了高水平分泌TGF-β2的CHO和COS转染子。 用携带完整的414个氨基酸猿TGF-β2前体的质粒载体转染的CHO细胞分泌约5μg/ ml培养基。

    Cloning and expression of a hybrid transforming growth factor-beta-1/transforming growth factor-beta-2
    5.
    发明公开
    Cloning and expression of a hybrid transforming growth factor-beta-1/transforming growth factor-beta-2 失效
    混合物：变形菌株Wachstumsfaktorβ-1 /转化酶Wachstumsfaktorβ-2。

    公开(公告)号：EP0676474A1

    公开(公告)日：1995-10-11

    申请号：EP95104223.3

    申请日：1989-12-14

    申请人： ONCOGEN LIMITED PARTNERSHIP

    发明人： Purchio, Anthony F., c/o Advanced Tissue Sciences ,  Madisen, Linda ,  Webb, Nancy

    IPC分类号： C12N15/62 ,  C12N15/85 ,  C07K14/495 ,  C12N5/10

    CPC分类号： C07K14/495 ,  A61K38/00 ,  C07K2319/00

    摘要： The present invention relates to hybrid TGF-β1/TGF-β2 precursor proteins, nucleotide sequences encoding the hybrid TGF-β1/β2 precursors, and methods of producing transforming growth factor-β2 using the nucleotide sequences encoding the hybrid precursors. Embodiments of the invention include hybrid TGF-β1/β2 precursors comprising the amino acid sequence substantially as depicted in Fig. 1b from about amino acid residue number 1 to about amino acid residue number 390 or from about amino acid residue number 30 to about amino acid residue number 390. In another embodiment, a nucleotide sequence of the invention comprises the nucleotide coding sequence substantially as depicted in Fig. 1b from about nucleotide residue number -70 to about nucleotide residue number 1755.

    摘要翻译： 本发明涉及杂交TGF-β1/ TGF-β2前体蛋白，编码杂合TGF-β1/β2前体的核苷酸序列，以及使用编码杂合前体的核苷酸序列产生转化生长因子-β2的方法 。 本发明的实施方案包括包含基本如图1所示的氨基酸序列的杂合TGF-β1/β2前体。 1b的约11个氨基酸残基，约氨基酸残基数390或约氨基酸残基数30至约氨基酸残基数390.在另一个实施方案中，本发明的核苷酸序列包含基本上如 图。 1b从约核苷酸残基数-70至约核苷酸残基数1755。