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公开(公告)号：EP3323896A2
公开(公告)日：2018-05-23
申请号：EP17183834.5
申请日：2012-01-30
申请人： Olink Proteomics AB
IPC分类号： C12Q1/68 , G01N33/542 , G01N33/58
摘要： The present invention relates to a proximity probe based detection assay ("proximity assay") for an analyte in a sample, specifically a proximity probe extension assay (PEA), an in particular to an improvement in the method to reduce non-specific "background" signals, wherein the improvement comprises the use in such assays of a component comprising 3' exonuclease activity, said method comprising: (a) contacting said sample with at least one set of at least first and second proximity probes, which probes each comprise an analyte-binding domain and a nucleic acid domain and can simultaneously bind to the analyte; (b) allowing the nucleic acid domains of the proximity probes to interact with each other upon binding of said proximity probes to said analyte, wherein said interaction comprises the formation of a duplex; (c) contacting said sample with a component comprising 3' exonuclease activity; (d) extending the 3' end of at least one nucleic acid domain of said duplex to generate an extension product, wherein the step may occur contemporaneously with or after step (c); and (e) amplifying and detecting the extension product.
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公开(公告)号：EP3323896B1
公开(公告)日：2020-05-27
申请号：EP17183834.5
申请日：2012-01-30
申请人： Olink Proteomics AB
IPC分类号： C12Q1/68 , G01N33/542 , G01N33/58
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公开(公告)号：EP2670860B1
公开(公告)日：2017-08-16
申请号：EP12702018.8
申请日：2012-01-30
申请人： Olink Proteomics AB
IPC分类号： C12Q1/68 , G01N33/542 , G01N33/58
CPC分类号： C12Q1/6848 , C12Q1/6804 , C12Q1/689 , C12Q2600/16 , G01N33/542 , G01N33/558 , C12Q2521/325 , C12Q2533/101
摘要： The present invention relates to a proximity probe based detection assay ("proximity assay") for an analyte in a sample, specifically a proximity probe extension assay (PEA), an in particular to an improvement in the method to reduce non-specific "background" signals, wherein the improvement comprises the use in such assays of a component comprising 3' exonuclease activity, said method comprising: (a) contacting said sample with at least one set of at least first and second proximity probes, which probes each comprise an analyte-binding domain and a nucleic acid domain and can simultaneously bind to the analyte; (b) allowing the nucleic acid domains of the proximity probes to interact with each other upon binding of said proximity probes to said analyte, wherein said interaction comprises the formation of a duplex; (c) contacting said sample with a component comprising 3' exonuclease activity; (d) extending the 3' end of at least one nucleic acid domain of said duplex to generate an extension product, wherein the step may occur contemporaneously with or after step (c); and (e) amplifying and detecting the extension product.
摘要翻译： 本发明涉及用于样品中分析物特别是邻近探针延伸测定（PEA）的基于邻近探针的检测测定（“邻近测定”），尤其涉及用于减少非特异性“背景 “信号，其中所述改进包括在这种测定中使用包含3'外切核酸酶活性的组分，所述方法包括：（a）使所述样品与至少一组至少第一和第二邻近探针接触，所述探针各自包含 分析物结合结构域和核酸结构域，并且可以同时结合分析物; （b）使所述邻近探针与所述分析物结合时，使所述邻近探针的核酸结构域彼此相互作用，其中所述相互作用包括形成双链体; （c）使所述样品与包含3'外切核酸酶活性的组分接触; （d）延伸所述双链体的至少一个核酸域的3'末端以产生延伸产物，其中所述步骤可以与步骤（c）同时或之后发生; 和（e）放大并检测延伸产物。
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公开(公告)号：EP3323896A3
公开(公告)日：2018-06-13
申请号：EP17183834.5
申请日：2012-01-30
申请人： Olink Proteomics AB
IPC分类号： C12Q1/68 , G01N33/542 , G01N33/58
摘要： The present invention relates to a proximity probe based detection assay ("proximity assay") for an analyte in a sample, specifically a proximity probe extension assay (PEA), an in particular to an improvement in the method to reduce non-specific "background" signals, wherein the improvement comprises the use in such assays of a component comprising 3' exonuclease activity, said method comprising: (a) contacting said sample with at least one set of at least first and second proximity probes, which probes each comprise an analyte-binding domain and a nucleic acid domain and can simultaneously bind to the analyte; (b) allowing the nucleic acid domains of the proximity probes to interact with each other upon binding of said proximity probes to said analyte, wherein said interaction comprises the formation of a duplex; (c) contacting said sample with a component comprising 3' exonuclease activity; (d) extending the 3' end of at least one nucleic acid domain of said duplex to generate an extension product, wherein the step may occur contemporaneously with or after step (c); and (e) amplifying and detecting the extension product.
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