公开(公告)号：EP3527986A1

公开(公告)日：2019-08-21

申请号：EP19153680.4

申请日：2019-01-25

申请人： Sumitomo Rubber Industries, Ltd. ,  Yamagata University ,  Osaka University

发明人： MINAGAWA, Yasuhisa ,  TANAKA, Masaru ,  HOSHIBA, Takashi ,  EMURA, Haruka ,  MORI, Masaki ,  YAMAMOTO, Hirofumi ,  HARAGUCHI, Naotsugu

IPC分类号： G01N33/543 ,  G01N33/574 ,  A61M1/36

摘要： The present invention provides a method for capturing specific cells (e.g. many types of cancer cells, including cancer cells not expressing EpCAM) and a method for analysis of specific cells involving the method. Included is a method for capturing specific cells present in blood or biological fluid, the method including: centrifuging sampled blood or biological fluid at a centrifugal force of 1000 to 3000 G; and capturing specific cells therefrom onto a hydrophilic polymer layer.

公开(公告)号：EP3527985A1

公开(公告)日：2019-08-21

申请号：EP19153318.1

申请日：2019-01-23

申请人： Sumitomo Rubber Industries, Ltd. ,  Yamagata University ,  Osaka University

发明人： MINAGAWA, Yasuhisa ,  TANAKA, Masaru ,  HOSHIBA, Takashi ,  EMURA, Haruka ,  MORI, Masaki ,  YAMAMOTO, Hirofumi ,  HARAGUCHI, Naotsugu

IPC分类号： G01N33/543 ,  G01N33/574

摘要： The present invention provides a method for capturing specific cells (e.g. many types of cancer cells, including cancer cells not expressing EpCAM) and a method for analysis of specific cells involving the method. Included is a method for capturing specific cells present in blood or biological fluid, the method including: subjecting sampled blood or biological fluid to enrichment; and capturing specific cells therefrom onto a hydrophilic polymer layer in a flow field.

公开(公告)号：EP2537930A1

公开(公告)日：2012-12-26

申请号：EP11744730.0

申请日：2011-02-18

申请人： Osaka University

发明人： MORI, Masaki ,  ISHII, Hideshi ,  MIYOSHI, Norikatsu ,  DOKI, Yuichiro ,  TANEMURA, Masahiro ,  NAGAI, Kenichi ,  HOSHINO, Hiromitsu ,  OMURA, Yoshiaki ,  HARAGUCHI, Naotsugu ,  MIYAZAKI, Susumu

IPC分类号： C12N15/09 ,  C12N5/10 ,  C12Q1/02 ,  C12Q1/68

CPC分类号： C12N5/0696 ,  C12N2501/65 ,  C12N2506/1307 ,  C12N2506/1384 ,  G01N33/5008

摘要： A major object of the present invention is to provide a method for producing induced pluripotent stem cells with low tumorigenesis potential and high induction efficiency.
The invention provides a method for producing induced pluripotent stem cells comprising the step of introducing one or more nucleic acids that facilitate expression of at least one gene selected from the group consisting of NANOG, SOX2, OCT3/4, KLF4, LIN28, and c-MYC into somatic cells.

摘要翻译： 本发明的主要目的是提供具有低致瘤潜力和诱导效率高的诱导多能干细胞的方法。 本发明提供一种产生诱导性多能干细胞的方法，包括引入一种或多种促进至少一种选自NANOG，SOX2，OCT3 / 4，KLF4，LIN28和c- MYC进入体细胞。