公开(公告)号：EP3613499A1

公开(公告)日：2020-02-26

申请号：EP19203649.9

申请日：2013-10-23

申请人： Oxford Nanopore Technologies Limited

发明人： HYDE, Jason Robert ,  BAHAMON, Pedro Miguel Ortiz ,  BROWN, Andrew John ,  HERON, Andrew John ,  MACKETT, Paul Raymond

IPC分类号： B01J19/00 ,  B01L3/00 ,  G01N33/487

摘要： An array of membranes comprising amphipathic molecules is formed using an apparatus comprising a support defining an array of compartments. Volumes comprising polar medium are provided within respective compartments and a layer comprising apolar medium is provided extending across the openings with the volumes. Polar medium is flowed across the support to displace apolar medium and form a layer in contact with the volumes, forming membranes comprising amphipathic molecules at the interfaces. In one construction of the apparatus, the support that comprises partitions which comprise inner portions and outer portions. The inner portions define inner recesses without gaps therebetween that are capable of constraining the volumes comprising polar medium contained in neighbouring inner recesses from contacting each other. The outer portions extend outwardly from the inner portions and have gaps allowing the flow of an apolar medium across the substrate.

公开(公告)号：EP2911781B1

公开(公告)日：2019-12-18

申请号：EP13785899.9

申请日：2013-10-23

申请人： Oxford Nanopore Technologies Limited

发明人： HYDE, Jason Robert ,  BAHAMON, Pedro Miguel Ortiz ,  BROWN, Clive Gavin ,  HERON, Andrew John ,  MACKETT, Paul Raymond

IPC分类号： B01J19/00 ,  B01L3/00 ,  G01N33/487

公开(公告)号：EP2911781A2

公开(公告)日：2015-09-02

申请号：EP13785899.9

申请日：2013-10-23

申请人： Oxford Nanopore Technologies Limited

发明人： HYDE, Jason Robert ,  BAHAMON, Pedro Miguel Ortiz ,  BROWN, Clive Gavin ,  HERON, Andrew John ,  MACKETT, Paul Raymond

IPC分类号： B01J19/00 ,  B01L3/00 ,  G01N33/487

摘要： An array of membranes comprising amphipathic molecules is formed using an apparatus comprising a support defining an array of compartments. Volumes comprising polar medium are provided within respective compartments and a layer comprising apolar medium is provided extending across the openings with the volumes. Polar medium is flowed across the support to displace apolar medium and form a layer in contact with the volumes, forming membranes comprising amphipathic molecules at the interfaces. In one construction of the apparatus, the support that comprises partitions which comprise inner portions and outer portions. The inner portions define inner recesses without gaps therebetween that are capable of constraining the volumes comprising polar medium contained in neighbouring inner recesses from contacting each other. The outer portions extend outwardly from the inner portions and have gaps allowing the flow of an apolar medium across the substrate.