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公开(公告)号：EP2994544A4
公开(公告)日：2017-01-25
申请号：EP14794438
申请日：2014-05-05
发明人： TURNER STEPHEN , HANES JEREMIAH , BJORNSON KEITH
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6874 , C12Q1/6823 , G01N27/227 , C12Q2521/101 , C12Q2521/543 , C12Q2535/122 , C12Q2563/113 , C12Q2563/116 , C12Q2563/157 , C12Q2565/607
摘要： Real time electronic sequencing methods, devices, and systems are described. Arrays of nanoscale electronic elements comprising capacitive devices with one or two electrodes, or arrays of nanoFET devices are used to provide sequence information about a template nucleic acid in a polymerase-template complex bound proximate to the nanoscale electronic elements or to the substrate proximate to the nanoscale electronic element. A sequencing reaction mixture comprising nucleotide analogs having impedance labels, capacitive labels, or conductivity labels is introduced to the array of nanoscale electronic elements comprising capacitive devices or nanoFETs under conditions of polymerase mediated nucleic acid synthesis. The time sequence of incorporation of nucleotide analogs is determined by identifying the types of labels of the nucleotide analogs that are incorporated into the growing strand using measured impedance, conductivity, or capacitance.
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公开(公告)号：EP3008094A4
公开(公告)日：2017-04-12
申请号：EP14810199
申请日：2014-06-12
发明人： MILLER ERIK , KAMTEKAR SATWIK , BJORNSON KEITH , HANES JEREMIAH
摘要： Multi-biotinylated reactants are provided which can be used in divalent complexes for various applications such as colocalization, labeling, immobilization, and purification. Methods for constructing, purifying, and using the bis-biotinylated reactants are also provided. In certain embodiments, two bis-biotinylated reactants are bound to a single streptavidin tetramer to provide a complex having a 1:1 stoichiometry with respect to the bis-biotinylated reactants.
摘要翻译： 提供多生物素化反应物，其可以用于二价复合物用于各种应用，例如共定位，标记，固定和纯化。 还提供了构建，纯化和使用双生物素化反应物的方法。 在某些实施方案中，两种双生物素化的反应物与单个链霉抗生物素蛋白四聚体结合以提供相对于双生物素化反应物具有1：1化学计量的复合物。
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