公开(公告)号：EP2994544A4

公开(公告)日：2017-01-25

申请号：EP14794438

申请日：2014-05-05

Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC

Inventor： TURNER STEPHEN ,  HANES JEREMIAH ,  BJORNSON KEITH

IPC: C12Q1/68

CPC classification number: C12Q1/6874 ,  C12Q1/6823 ,  G01N27/227 ,  C12Q2521/101 ,  C12Q2521/543 ,  C12Q2535/122 ,  C12Q2563/113 ,  C12Q2563/116 ,  C12Q2563/157 ,  C12Q2565/607

Abstract: Real time electronic sequencing methods, devices, and systems are described. Arrays of nanoscale electronic elements comprising capacitive devices with one or two electrodes, or arrays of nanoFET devices are used to provide sequence information about a template nucleic acid in a polymerase-template complex bound proximate to the nanoscale electronic elements or to the substrate proximate to the nanoscale electronic element. A sequencing reaction mixture comprising nucleotide analogs having impedance labels, capacitive labels, or conductivity labels is introduced to the array of nanoscale electronic elements comprising capacitive devices or nanoFETs under conditions of polymerase mediated nucleic acid synthesis. The time sequence of incorporation of nucleotide analogs is determined by identifying the types of labels of the nucleotide analogs that are incorporated into the growing strand using measured impedance, conductivity, or capacitance.

公开(公告)号：EP3332032A1

公开(公告)日：2018-06-13

申请号：EP16833794

申请日：2016-08-03

Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC

Inventor： TURNER STEPHEN ,  KORLACH JONAS ,  WARREN STEVEN

IPC: C12Q1/68 ,  B01L3/00 ,  C12M1/34 ,  G01N27/22 ,  H01L31/109

CPC classification number: C12Q1/6869 ,  B82Y10/00 ,  B82Y15/00 ,  G01N27/4145 ,  G01N27/4146 ,  G01N27/4148 ,  G11C19/28 ,  H01L29/0669 ,  H01L29/0673 ,  H01L29/1606

Abstract: A circuit comprising a substrate with sectors on the substrate is provided, each sector comprising clock and data lines, a controller in electrical communication with the clock and data lines, a counter bias line, an amplifier input line and nano-electronic measurement devices on the substrate. A source of each device is coupled to the counter bias line and a drain of each device is coupled to the amplifier input line to obtain an electrical signal on the drain, the identity of which is determined by electrical interaction between the device and a charge label. Each device drain is gated by a corresponding switch between an on state, in which the drain is connected to the amplifier input line, and an off state, in which the drain is isolated from the amplifier input line. The controller controls switch states responsive to clock signal line pulses and data input line data.