公开(公告)号：EP0835446B1

公开(公告)日：2003-04-09

申请号：EP96923455.8

申请日：1996-06-26

申请人： PERSEPTIVE BIOSYSTEMS, INC.

发明人： JINDAL, Satish ,  REGNIER, Fred, E. ,  WILLIAMS, Kevin ,  AFEYAN, Noubar, B. ,  PALIWAL, Sandeep ,  EVANS, David ,  PINGALI, Aruna

IPC分类号： G01N30/46

CPC分类号： G01N30/461 ,  G01N30/463 ,  G01N30/7233 ,  G01N30/88 ,  G01N2030/027 ,  G01N2030/8804 ,  G01N2030/8813 ,  G01N2030/8831

公开(公告)号：EP0835446A2

公开(公告)日：1998-04-15

申请号：EP96923455.0

申请日：1996-06-26

申请人： PERSEPTIVE BIOSYSTEMS, INC.

发明人： JINDAL, Satish ,  REGNIER, Fred, E. ,  WILLIAMS, Kevin ,  AFEYAN, Noubar, B. ,  PALIWAL, Sandeep ,  EVANS, David ,  PINGALI, Aruna

IPC分类号： G01N27 ,  B01J20 ,  C12Q1 ,  C40B20 ,  C40B30 ,  C40B40 ,  C40B50 ,  C40B60 ,  G01N30 ,  G01N33

CPC分类号： G01N30/461 ,  G01N30/463 ,  G01N30/7233 ,  G01N30/88 ,  G01N2030/027 ,  G01N2030/8804 ,  G01N2030/8813 ,  G01N2030/8831

摘要： The invention provides novel methods for screening a sample to select a ligand to a target of interest and for obtaining information about the ligand and its binding characteristics. Specifically, the claimed multi-dimensional methods involve combining a solution of heterogeneous ligands with the target of interest to screen the ligands on the basis of one or more binding characteristics. Ligands having the first binding characteristic bind to the target of interest thereby to form a target/ligand complex. The complex then optionally is separated from the unbound components using any of a variety of separation techniques, e.g., size exclusion. At least one of the complex or unbound components then is introduced to a second 'dimension'. The second dimension is capable of separating components based upon a second binding characteristic. One then elutes the ligand having the desired binding characteristics.