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公开(公告)号：EP0751823B1
公开(公告)日：1999-11-03
申请号：EP95911918.1
申请日：1995-02-24
发明人： AFEYAN, Noubar, B. , REGNIER, Fred, E. , MU, Ning
CPC分类号： B01D15/08 , B01J20/261 , B01J20/264 , B01J20/265 , B01J20/267 , B01J20/28004 , B01J20/28078 , B01J20/286 , B01J20/3092 , B01J20/321 , B01J20/3217 , B01J20/327 , B01J20/3282 , B01J2220/54 , B01J2220/58 , Y10T428/2998
摘要： Disclosed are hydrophilic coatings covalently bound to hydrophobic surfaces as well as methods for their production. To form the coated surfaces, a compound is provided which comprises a hydrophobic domain including an unsaturated group and a hydrophilic domain. Also provided is a hydrophobic surface comprising unsaturated groups. Molecules of the compound are adsorbed onto the hydrophobic surface, and the unsaturated groups in the hydrophobic domains of molecules of the compound then are covalently crosslinked to the unsaturated groups on the hydrophobic surface by a free radical reaction. In one embodiment, hydrophilic coatings may be covalently attached to hydrophobic polymers such as divinylbenzene crosslinked polystyrene. Hydrophilic coatings covalently attached to hydrophobic surfaces are obtained which are stable, which may be readily derivatized, and which are useful in a wide range of chromotography applications.
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公开(公告)号：EP0632895B1
公开(公告)日：1998-07-08
申请号：EP93908606.2
申请日：1993-03-26
IPC分类号： G01N35/00 , G01N33/543 , G01N33/546
CPC分类号： G01N33/54366 , G01N30/02 , G01N30/52 , G01N2030/324 , G01N2030/525 , G01N2030/8813 , G01N2030/8822 , G01N2030/8831 , B01D15/3804
摘要： Disclosed is an apparatus and method for the rapid flowthrough assay of an analyte. A multiplicity of binding sites which bind selectively to an analyte are immobilized on a rigid matrix, which defines a flowpath. A liquid sample containing the analyte is delivered convectively to a hydraulically full volume through the matrix to permit formation of a binding site: analyte complex. A detectable tagging reagent capable of binding with the binding site or the complex is then delivered convectively to the matrix and the amount of tagging reagent which is bound is detected to provide an assay of the concentration or presence of the analyte.
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公开(公告)号：EP0663073A1
公开(公告)日：1995-07-19
申请号：EP93921604.0
申请日：1993-09-15
CPC分类号： C12Q1/60 , G01N33/92 , G01N2800/044 , Y10T436/255
摘要： Disclosed is a method for the rapid quantitative determination of low density lipoprotein (LDL) in a biological fluid sample containing LDL, very low density lipoprotein (VLDL) and high density lipoprotein (HDL). The sample is first passed through a matrix to bind LDL to the matrix. The LDL is then eluted from the matrix diferentially from VLDL and HDL. A parameter representative of the concentration of a solute exiting the matrix during the interval when LDL is eluted is then determined, wherein the parameter is proportional to the amount of the LDL in the sample. The parameter may be determined, e.g., by electronically integrating under a spectrophotometrically detected peak of eluted LDL.
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公开(公告)号：EP0633811A1
公开(公告)日：1995-01-18
申请号：EP93908469.0
申请日：1993-03-23
CPC分类号： G01N33/54353 , B01J20/321 , B01J20/3219 , B01J20/3248 , B01J20/3251 , B01J20/3285 , G01N33/545 , G01N2600/00
摘要： Disclosed are chemically-produced specific binding, 'molecular imaged' sorbents which reversibly bind a preselected macromolecule by spacially matched multipoint interactions between functional groups synthesized on the surface of the sorbent and functional groups on the surface of the macromolecule. Also disclosed are methods of producing such sorbents. The sorbents typically are high surface area solids comprising surface binding regions which have charged groups, metal coordinating groups, hydrophobic moieties, or various combinations thereof anchored thereto and spaced in the mirror image of complementary interactive groups on a surface of the macromolecule.
摘要翻译： 公开了化学产生的特异性结合“分子成像”吸附剂，其通过在吸附剂表面上合成的官能团和大分子表面上的官能团之间的空间匹配的多点相互作用可逆地结合预选的大分子。 还公开了生产这种吸附剂的方法。 吸附剂通常是高表面积固体，其包含表面结合区域，该表面结合区域具有带电荷基团，金属配位基团，疏水性单元或其锚定于其上并在大分子表面上的互补相互作用基团的镜像中间隔开的各种组合。
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5.发明授权HIGH SPEED, AUTOMATED, CONTINUOUS FLOW, MULTI-DIMENSIONAL MOLECULAR SELECTION AND ANALYSIS 失效自动，连续进行多维度的分子VELOCITY选择和分析
公开(公告)号：EP0835446B1
公开(公告)日：2003-04-09
申请号：EP96923455.8
申请日：1996-06-26
发明人： JINDAL, Satish , REGNIER, Fred, E. , WILLIAMS, Kevin , AFEYAN, Noubar, B. , PALIWAL, Sandeep , EVANS, David , PINGALI, Aruna
IPC分类号： G01N30/46
CPC分类号： G01N30/461 , G01N30/463 , G01N30/7233 , G01N30/88 , G01N2030/027 , G01N2030/8804 , G01N2030/8813 , G01N2030/8831
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6.发明公开HIGH SPEED, AUTOMATED, CONTINUOUS FLOW, MULTI-DIMENSIONAL MOLECULAR SELECTION AND ANALYSIS 失效自动，连续进行多维度的分子VELOCITY选择和分析
公开(公告)号：EP0835446A2
公开(公告)日：1998-04-15
申请号：EP96923455.0
申请日：1996-06-26
发明人： JINDAL, Satish , REGNIER, Fred, E. , WILLIAMS, Kevin , AFEYAN, Noubar, B. , PALIWAL, Sandeep , EVANS, David , PINGALI, Aruna
CPC分类号： G01N30/461 , G01N30/463 , G01N30/7233 , G01N30/88 , G01N2030/027 , G01N2030/8804 , G01N2030/8813 , G01N2030/8831
摘要： The invention provides novel methods for screening a sample to select a ligand to a target of interest and for obtaining information about the ligand and its binding characteristics. Specifically, the claimed multi-dimensional methods involve combining a solution of heterogeneous ligands with the target of interest to screen the ligands on the basis of one or more binding characteristics. Ligands having the first binding characteristic bind to the target of interest thereby to form a target/ligand complex. The complex then optionally is separated from the unbound components using any of a variety of separation techniques, e.g., size exclusion. At least one of the complex or unbound components then is introduced to a second 'dimension'. The second dimension is capable of separating components based upon a second binding characteristic. One then elutes the ligand having the desired binding characteristics.
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公开(公告)号：EP0751823A1
公开(公告)日：1997-01-08
申请号：EP95911918.0
申请日：1995-02-24
发明人： AFEYAN, Noubar, B. , REGNIER, Fred, E. , MU, Ning
CPC分类号： B01D15/08 , B01J20/261 , B01J20/264 , B01J20/265 , B01J20/267 , B01J20/28004 , B01J20/28078 , B01J20/286 , B01J20/3092 , B01J20/321 , B01J20/3217 , B01J20/327 , B01J20/3282 , B01J2220/54 , B01J2220/58 , Y10T428/2998
摘要： Disclosed are hydrophilic coatings covalently bound to hydrophobic surfaces as well as methods for their production. To form the coated surfaces, a compound is provided which comprises a hydrophobic domain including an unsaturated group and a hydrophilic domain. Also provided is a hydrophobic surface comprising unsaturated groups. Molecules of the compound are adsorbed onto the hydrophobic surface, and the unsaturated groups in the hydrophobic domains of molecules of the compound then are covalently crosslinked to the unsaturated groups on the hydrophobic surface by a free radical reaction. In one embodiment, hydrophilic coatings may be covalently attached to hydrophobic polymers such as divinylbenzene crosslinked polystyrene. Hydrophilic coatings covalently attached to hydrophobic surfaces are obtained which are stable, which may be readily derivatized, and which are useful in a wide range of chromotography applications.
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公开(公告)号：EP0670999A1
公开(公告)日：1995-09-13
申请号：EP94902406.0
申请日：1993-11-23
CPC分类号： G01N27/44795 , G01N27/44747
摘要： Disclosed are methods and apparatus for determining the presence of one or more analytes in a sample, wherein the presence of a complex of an analyte and an analyte-specific binding moiety is detected at a location in an elongate pH gradient corresponding to a predetermined isoelectric point of the complex in the gradient. An electric field applied across the elongate pH gradient prior to the detection of the complex transports the complex to the location in the pH gradient corresponding to the predetermined isoelectric point. The analyte-specific binding moiety preferably is provided with a detectable label such as a fluorescent label. A parameter, e.g., fluorescence intensity, indicative of the amount of the complex at the location in the pH gradient corresponding to the predetermined isoelectric point may be determined to quantitate the analyte.
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公开(公告)号：EP0632895A1
公开(公告)日：1995-01-11
申请号：EP93908606.0
申请日：1993-03-26
CPC分类号： G01N33/54366 , G01N30/02 , G01N30/52 , G01N2030/324 , G01N2030/525 , G01N2030/8813 , G01N2030/8822 , G01N2030/8831 , B01D15/3804
摘要： Disclosed is an apparatus and method for the rapid flowthrough assay of an analyte. A multiplicity of binding sites which bind selectively to an analyte are immobilized on a rigid matrix, which defines a flowpath. A liquid sample containing the analyte is delivered convectively to a hydraulically full volume through the matrix to permit formation of a binding site: analyte complex. A detectable tagging reagent capable of binding with the binding site or the complex is then delivered convectively to the matrix and the amount of tagging reagent which is bound is detected to provide an assay of the concentration or presence of the analyte.
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10.发明公开ON-LINE PRODUCT IDENTIFICATION IN A CHROMATOGRAPHY EFFLUENT BY SUBTRACTION 失效CHROMATOGRAPHISCHEM EFFLUENTEN DURCH DIFFERENZBILDUNG的制备方法。
公开(公告)号：EP0533909A1
公开(公告)日：1993-03-31
申请号：EP92910887.0
申请日：1992-03-20
CPC分类号： G01N30/461 , B01D15/08 , G01N27/44717 , G01N30/62 , G01N30/82 , G01N2030/143 , G01N2030/524 , G01N2030/625 , G01N2030/8813 , G01N2030/8831
摘要： Procédé et appareil servant à l'identification rapide d'un soluté à examiner dans une phase fluide en sortie (14). Le procédé consiste à séparer les solutés dans la phase fluide (14) et à identifier un soluté prédéterminé par sa soustraction sélective de la phase fluide (14). On fait passer dans un détecteur (16) la phase fluide (14) sortant d'un premier système (12) apte à partager les constituants du mélange, de manière à obtenir un premier signal de sortie (18) décrivant la séquence temporelle et/ou spatiale des constituants du mélange sortant du premier système (12). Ensuite, on fait passer ladite phase fluide (14) dans un second système (20) apte à en extraire de manière sélective un soluté à examiner, puis on la fait passer dans un détecteur (16).
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