公开(公告)号：EP0730602A1

公开(公告)日：1996-09-11

申请号：EP95902082.0

申请日：1994-11-22

申请人： PNA Diagnostics A/S

发明人： STANLEY, Christopher, John ,  ORUM, Henrik ,  JORGENSEN, Mikkel

IPC分类号： G01N33 ,  C07H21 ,  C07K14 ,  C08G69 ,  C08G75 ,  C09K3 ,  C12Q1

CPC分类号： C07K14/003 ,  C07H21/00 ,  C12Q1/6813

摘要： A nucleic acid analog comprising a polymeric strand which includes a sequence of ligands bound to a backbone made up of linked backbone moieties, which analog is capable of hybridization to a nucleic acid of complementary sequence, further comprising a chelating moiety capable of binding at least one metal ion by chelation.

公开(公告)号：EP0730602B1

公开(公告)日：1998-08-12

申请号：EP95902082.7

申请日：1994-11-22

申请人： PNA Diagnostics A/S

发明人： STANLEY, Christopher, John ,  ORUM, Henrik ,  JORGENSEN, Mikkel

IPC分类号： C07H21/00 ,  C07K14/00 ,  C12Q1/68

CPC分类号： C07K14/003 ,  C07H21/00 ,  C12Q1/6813

摘要： A nucleic acid analog comprising a polymeric strand which includes a sequence of ligands bound to a backbone made up of linked backbone moieties, which analog is capable of hybridization to a nucleic acid of complementary sequence, further comprising a chelating moiety capable of binding at least one metal ion by chelation.

公开(公告)号：EP0733062A1

公开(公告)日：1996-09-25

申请号：EP95903782.0

申请日：1994-11-30

申请人： PNA Diagnostics A/S

发明人： STANLEY, Christopher, John ,  ORUM, Henrik ,  JORGENSEN, Mikkel ,  BASBOLL, Ole

IPC分类号： G01N33 ,  C07H21 ,  C07K14 ,  C12N15 ,  C12Q1

CPC分类号： C07K14/003 ,  C07H21/00 ,  C12Q1/6816 ,  C12Q2525/107 ,  C12Q2521/319 ,  C12Q2521/301

摘要： A selected region of a nucleic acid is protected from attack by nuclease by complexing to the nucleic acid a nucleic acid analogue of the PNA type. The surviving sequence may be detected in an assay, optionally after amplification. A PCR reaction may be sterilised and its product assayed by protecting a characterising region of the product by PNA hybridisation followed by nuclease degradation of unprotected nucleic acid.

公开(公告)号：EP0733062B1

公开(公告)日：1998-05-13

申请号：EP95903782.1

申请日：1994-11-30

申请人： PNA Diagnostics A/S

发明人： STANLEY, Christopher, John ,  ORUM, Henrik ,  JORGENSEN, Mikkel ,  BASBOLL, Ole

IPC分类号： C07K14/00 ,  C12Q1/68 ,  C07H21/00

CPC分类号： C07K14/003 ,  C07H21/00 ,  C12Q1/6816 ,  C12Q2525/107 ,  C12Q2521/319 ,  C12Q2521/301

摘要： A selected region of a nucleic acid is protected from attack by nuclease by complexing to the nucleic acid a nucleic acid analogue of the PNA type. The surviving sequence may be detected in an assay, optionally after amplification. A PCR reaction may be sterilised and its product assayed by protecting a characterising region of the product by PNA hybridisation followed by nuclease degradation of unprotected nucleic acid.