公开(公告)号：EP3124118B1

公开(公告)日：2018-02-28

申请号：EP16170761.7

申请日：2016-05-23

申请人： Panasonic Intellectual Property Management Co., Ltd.

发明人： Hishida, Mitsuoki

IPC分类号： B01L7/00 ,  B01L3/00 ,  C12Q1/68

CPC分类号： B01L7/52 ,  B01L3/502715 ,  B01L2200/027 ,  B01L2300/0816 ,  B01L2300/0874 ,  B01L2300/0887 ,  B01L2300/1822 ,  B01L2400/0655 ,  C12P19/34 ,  C12Q1/686 ,  C12Q2547/101

摘要： A method for performing a polymerase chain reaction (PCR) process on a treatment target liquid using a microchannel chip and a heating/cooling control apparatus is provided. The microchannel chip includes: a first substrate layer that has an introducing channel and a discharging channel; and a second substrate layer that is disposed on the first substrate layer and has a second substrate layer body and a metal film, the second substrate layer body having a microchannel connected to the two channels, the metal film structuring an upper surface of the microchannel. The heating/cooling control apparatus includes: a temperature regulator; a power source that supplies voltage to the temperature regulator; and a heating/cooling controller that controls heating or cooling of the temperature regulator by controlling the voltage. The heating/cooling controller controls the voltage to change the temperature of the temperature regulator. The heat of the temperature regulator is conducted to the liquid in the microchannel via the metal film, thereby achieving the PCR process.

公开(公告)号：EP3124118A1

公开(公告)日：2017-02-01

申请号：EP16170761.7

申请日：2016-05-23

申请人： Panasonic Intellectual Property Management Co., Ltd.

发明人： Hishida, Mitsuoki

IPC分类号： B01L7/00 ,  B01L3/00 ,  C12Q1/68

CPC分类号： B01L7/52 ,  B01L3/502715 ,  B01L2200/027 ,  B01L2300/0816 ,  B01L2300/0874 ,  B01L2300/0887 ,  B01L2300/1822 ,  B01L2400/0655 ,  C12P19/34 ,  C12Q1/686 ,  C12Q2547/101

摘要： A method for performing a polymerase chain reaction (PCR) process on a treatment target liquid using a microchannel chip and a heating/cooling control apparatus is provided. The microchannel chip includes: a first substrate layer that has an introducing channel and a discharging channel; and a second substrate layer that is disposed on the first substrate layer and has a second substrate layer body and a metal film, the second substrate layer body having a microchannel connected to the two channels, the metal film structuring an upper surface of the microchannel. The heating/cooling control apparatus includes: a temperature regulator; a power source that supplies voltage to the temperature regulator; and a heating/cooling controller that controls heating or cooling of the temperature regulator by controlling the voltage. The heating/cooling controller controls the voltage to change the temperature of the temperature regulator. The heat of the temperature regulator is conducted to the liquid in the microchannel via the metal film, thereby achieving the PCR process.

摘要翻译： 提供了使用微通道芯片和加热/冷却控制装置对处理目标液体进行聚合酶链式反应（PCR）方法的方法。 微通道芯片包括：具有引入通道和放电通道的第一衬底层; 以及第二基板层，其设置在所述第一基板层上并具有第二基板层主体和金属膜，所述第二基板层主体具有连接到所述两个通道的微通道，所述金属膜构成所述微通道的上表面。 加热/冷却控制装置包括：温度调节器; 将电压提供给温度调节器的电源; 以及通过控制电压来控制温度调节器的加热或冷却的加热/冷却控制器。 加热/冷却控制器控制电压来改变温度调节器的温度。 温度调节器的热量通过金属膜传导到微通道中的液体，从而实现PCR过程。