公开(公告)号：EP3670667A1

公开(公告)日：2020-06-24

申请号：EP18306741.2

申请日：2018-12-19

申请人： Paris Sciences et Lettres - Quartier Latin ,  CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE ,  Institut Curie ,  The President and Fellows of Harvard College ,  HiFiBiO (HK) Limited ,  Ecole Supérieure de Physique et de Chimie Industrielles de la Ville de Paris

发明人： GRIFFITHS, Andrew ,  AMIGORENA, Sebastien ,  LANTZ, Olivier ,  WEITZ, David ,  NGHE, Philippe ,  GERARD, Annabelle

IPC分类号： C12Q1/6806 ,  C12N15/10

摘要： The invention concerns a method for identifying cognate pairs of a ligand species and a receptor species, comprising co-compartmentalising ligand species and receptor species to form a set of microreactors, each microreactor comprising at least one ligand species and preferably at least one receptor species; assaying the recognition between ligands and receptors in each microreactor and based on this assay, classifying each microreactor as positive when at least one ligand species and at least one receptor species in the microreactor recognize one with the other or negative when no ligand species and no receptor species recognize in the microreactor; identifying ligand species and receptor species contained in each positive microreactor; establishing a subset of positive microreactors containing the same receptor species; determining the probability that in a given subset of positive microreactors containing the same receptor species, the ligand species recognizing the receptor species corresponds to the most frequent co-compartmentalized ligand species; and if the determined probability is greater than a predetermined threshold, identifying as a cognate pair the receptor species and the most frequent co-compartmentalized ligand species.

公开(公告)号：EP3899027A1

公开(公告)日：2021-10-27

申请号：EP19828270.9

申请日：2019-12-19

申请人： Paris Sciences et Lettres ,  Centre national de la recherche scientifique ,  Institut Curie ,  The President and Fellows of Harvard College ,  HiFiBiO (HK) Limited ,  Ecole Supérieure de Physique et de Chimie Industrielles de la Ville de Paris ,  INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE - INSERM

发明人： GRIFFITHS, Andrew ,  AMIGORENA, Sebastien ,  LANTZ, Olivier ,  WEITZ, David ,  NGHE, Philippe ,  GERARD, Annabelle

IPC分类号： C12Q1/6806 ,  C12N15/10

公开(公告)号：EP4234712A3

公开(公告)日：2023-09-27

申请号：EP23163171.4

申请日：2019-12-19

申请人： Paris Sciences et Lettres ,  Centre national de la recherche scientifique ,  Institut Curie ,  The President and Fellows of Harvard College ,  HiFiBiO (HK) Limited ,  Ecole Supérieure de Physique et de Chimie Industrielles de la Ville de Paris ,  INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE - INSERM

发明人： GRIFFITHS, Andrew ,  AMIGORENA, Sebastien ,  LANTZ, Olivier ,  WEITZ, David ,  GERARD, Annabelle ,  NGHE, Philippe

IPC分类号： C12Q1/6806 ,  C12N15/10

摘要： The invention concerns a method for identifying cognate pairs of a ligand species and a receptor species, comprising co-compartmentalising ligand species and receptor species to form a set of microreactors, each microreactor comprising at least one ligand species and preferably at least one receptor species; assaying the recognition between ligands and receptors in each microreactor and based on this assay, classifying each microreactor as positive when at least one ligand species and at least one receptor species in the microreactor recognize one with the other or negative when no ligand species and no receptor species recognize in the microreactor; identifying ligand species and receptor species contained in each positive microreactor; establishing a subset of positive microreactors containing the same receptor species; determining the probability that in a given subset of positive microreactors containing the same receptor species, the ligand species recognizing the receptor species corresponds to the most frequent co-compartmentalized ligand species; and if the determined probability is greater than a predetermined threshold, identifying as a cognate pair the receptor species and the most frequent co-compartmentalized ligand species.

公开(公告)号：EP4234712A2

公开(公告)日：2023-08-30

申请号：EP23163171.4

申请日：2019-12-19

申请人： Paris Sciences et Lettres ,  Centre national de la recherche scientifique ,  Institut Curie ,  The President and Fellows of Harvard College ,  HiFiBiO (HK) Limited ,  Ecole Supérieure de Physique et de Chimie Industrielles de la Ville de Paris ,  INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE - INSERM

发明人： GRIFFITHS, Andrew ,  AMIGORENA, Sebastien ,  LANTZ, Olivier ,  WEITZ, David ,  GERARD, Annabelle ,  NGHE, Philippe

IPC分类号： C12Q1/6806 ,  C12N15/10

摘要： The invention concerns a method for identifying cognate pairs of a ligand species and a receptor species, comprising co-compartmentalising ligand species and receptor species to form a set of microreactors, each microreactor comprising at least one ligand species and preferably at least one receptor species; assaying the recognition between ligands and receptors in each microreactor and based on this assay, classifying each microreactor as positive when at least one ligand species and at least one receptor species in the microreactor recognize one with the other or negative when no ligand species and no receptor species recognize in the microreactor; identifying ligand species and receptor species contained in each positive microreactor; establishing a subset of positive microreactors containing the same receptor species; determining the probability that in a given subset of positive microreactors containing the same receptor species, the ligand species recognizing the receptor species corresponds to the most frequent co-compartmentalized ligand species; and if the determined probability is greater than a predetermined threshold, identifying as a cognate pair the receptor species and the most frequent co-compartmentalized ligand species.

公开(公告)号：EP4278007A1

公开(公告)日：2023-11-22

申请号：EP22700092.4

申请日：2022-01-12

申请人： Paris Sciences et Lettres ,  Ecole Supérieure de Physique et de Chimie Industrielles de la Ville de Paris ,  Centre National de la Recherche Scientifique

发明人： GRIFFITHS, Andrew ,  BLIVET-BAILLY, Gaël ,  DE REYNIÈS, Aurélien ,  GEISLER, Hubert ,  IBANEZ, Pablo ,  NGHE, Philippe

IPC分类号： C12Q1/6837 ,  C12Q1/6841