公开(公告)号：EP3050976B1

公开(公告)日：2018-01-17

申请号：EP16154383.0

申请日：2008-11-24

申请人： PerkinElmer Health Sciences, Inc.

发明人： Adler, Karl Edwin, Jr. ,  Schermer, Mack J.

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6811 ,  C12Q1/6809 ,  C12Q1/6813 ,  C12Q1/6876 ,  Y10T436/143333 ,  C12Q2563/149 ,  C12Q2537/143 ,  C12Q2531/113 ,  C12Q2525/179 ,  C12Q2539/115

摘要： Encoded bead multiplex assays for chromosomal gains and losses are provided that provide the benefits of complex, large template DNA sources, such as BAC DNA, as the probe material without bead networking or other assay performance problems. Reagents for assaying DNA are described herein which include a plurality of encoded particles having attached amplicons amplified from a template DNA sequence. Each individual attached amplicon includes a nucleic acid sequence identical to a random portion of the template DNA sequence, wherein the amplicons together represent substantially the entire template DNA and wherein the nucleic acid sequence identical to a random portion of the template DNA sequence of each individual amplicon is shorter than the entire template DNA.

公开(公告)号：EP3050976A1

公开(公告)日：2016-08-03

申请号：EP16154383.0

申请日：2008-11-24

申请人： PerkinElmer Health Sciences, Inc.

发明人： Adler, Karl Edwin Jr. ,  Schermer, Mack J.

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6811 ,  C12Q1/6809 ,  C12Q1/6813 ,  C12Q1/6876 ,  Y10T436/143333 ,  C12Q2563/149 ,  C12Q2537/143 ,  C12Q2531/113 ,  C12Q2525/179 ,  C12Q2539/115

摘要： Encoded bead multiplex assays for chromosomal gains and losses are provided that provide the benefits of complex, large template DNA sources, such as BAC DNA, as the probe material without bead networking or other assay performance problems. Reagents for assaying DNA are described herein which include a plurality of encoded particles having attached amplicons amplified from a template DNA sequence. Each individual attached amplicon includes a nucleic acid sequence identical to a random portion of the template DNA sequence, wherein the amplicons together represent substantially the entire template DNA and wherein the nucleic acid sequence identical to a random portion of the template DNA sequence of each individual amplicon is shorter than the entire template DNA.

摘要翻译： 提供用于染色体增益和损失的编码珠多重测定法，其提供复杂的大模板DNA来源（例如BAC DNA）作为无珠网络或其他测定性能问题的探针材料的益处。 本文描述了用于测定DNA的试剂，其包括从模板DNA序列扩增的具有连接的扩增子的多个编码的颗粒。 每个单独连接的扩增子包括与模板DNA序列的随机部分相同的核酸序列，其中扩增子一起基本上代表整个模板DNA，并且其中与每个单独扩增子的模板DNA序列的随机部分相同的核酸序列 比整个模板DNA短。