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    PROCESS FOR THE ENZYMATIC PREPARATION OF A PRODUCT GLUCOSIDE AND OF A CO-PRODUCT FROM AN EDUCT GLUCOSIDE
    3.
    发明公开
    PROCESS FOR THE ENZYMATIC PREPARATION OF A PRODUCT GLUCOSIDE AND OF A CO-PRODUCT FROM AN EDUCT GLUCOSIDE 审中-公开
    用于酶解制备产品葡糖苷和来自教学用葡萄糖苷的副产品的方法

    公开(公告)号：EP3191598A1

    公开(公告)日：2017-07-19

    申请号：EP15760467.9

    申请日：2015-09-10

    申请人： Pfeifer & Langen GmbH & Co. KG

    发明人： KOCH, Timo Johannes ,  HÄSSLER, Thomas ,  KIPPING, Florian

    IPC分类号： C12P19/02 ,  C07H3/04 ,  C12P19/12 ,  C13K11/00 ,  C12N9/10

    CPC分类号： C12N9/1051 ,  C12P19/02 ,  C12P19/12 ,  C12P19/18 ,  C12Y204/0102 ,  C13K1/02 ,  C13K11/00

    摘要： The invention relates to a cellobiose phosphorylase, which catalyzes, among other things, the synthesis of cellobiose from glucose 1-phosphate and glucose. The cellobiose phosphorylase according to the invention can be understood as a mutation of the cellobiose phosphorylase from Cellulomonas uda. In comparison to cellobiose phosphorylase of the wild type, the cellobiose phosphorylase according to the invention is distinguished by improved activity and process stability, in particular temperature stability, and lower product inhibition and therefore is especially suitable for use in industrial processes.

    摘要翻译： 本发明涉及制备产物葡糖苷，优选寡糖，特别是纤维二糖，以及来自离析物葡糖苷，优选来自寡糖，尤其是来自寡糖的共产物，优选单糖，特别是果糖的制备方法 蔗糖，用酶催化。 由此首先将离析物葡糖苷酶解成葡萄糖1-磷酸和副产物，然后使葡萄糖1-磷酸酯反应以产生产物葡糖苷。 优选添加用于葡萄糖1-磷酸的反应物，优选另外的糖，特别是葡萄糖。 在离析物葡糖苷裂解中形成的副产物和在葡萄糖1-磷酸酯反应中形成的产物葡糖苷优选分别被分离。