公开(公告)号：EP3377613A1

公开(公告)日：2018-09-26

申请号：EP16801312.6

申请日：2016-11-10

申请人： Pfizer Inc.

发明人： DESAI, Sunil Gururao ,  HANSON, Michael Allen ,  KINROSS, Jonathan Patrick ,  LASKO, Daniel R ,  LOMBERK, Scott Ellis ,  LOTVIN, Jason Arnold ,  PATEL-BROWN, Sujata Kaushikbhai ,  SUN, Weiqiang ,  TOMASELLO, Peter Anthony

IPC分类号： C12N1/20 ,  C12R1/46

CPC分类号： C12N1/20 ,  C12P19/04 ,  C12R1/46

摘要： The present invention relates to media and fermentation methods for producing polysaccharides in bacterial cell culture. In one aspect, the invention relates to a complex culture medium comprising a vegetable hydrolysate, a yeast extract, and a carbon source. In another aspect, the invention relates to a defined media having a total amino acid concentration greater than about 50 mM. A further aspect of the invention relates to the use of fed batch and perfusion fermentation methods for cultivating polysaccharide- producing bacteria.