公开(公告)号：EP3912990A1

公开(公告)日：2021-11-24

申请号：EP21171606.3

申请日：2013-10-07

申请人： Pfizer Inc.

发明人： JANSEN, Kathrin Ute ,  ANDERSON, Annaliesa Sybil ,  DONALD, Robert G.K. ,  FLINT, Michael James ,  KALYAN, Narender Kumar ,  LOTVIN, Jason Arnold ,  SIDHU, Maninder K ,  MORAN, Justin Keith ,  RUPPEN, Mark Edward ,  SUN, Weiqiang

IPC分类号： C07K14/33 ,  A61K39/08

摘要： In one aspect, the invention relates to an immunogenic composition that includes a mutant Clostridium difficile toxin A and/or a mutant Clostridium difficile toxin B. The mutant toxin may include a glucosyltransferase domain having at least one mutation and a cysteine protease domain having at least one mutation, relative to the corresponding wild-type C. difficile toxin. The mutant toxins may include at least one amino acid that is chemically crosslinked. In another aspect, the invention relates to methods and compositions for use in culturing Clostridium difficile and in producing C. difficile toxins.

公开(公告)号：EP3377613A1

公开(公告)日：2018-09-26

申请号：EP16801312.6

申请日：2016-11-10

申请人： Pfizer Inc.

发明人： DESAI, Sunil Gururao ,  HANSON, Michael Allen ,  KINROSS, Jonathan Patrick ,  LASKO, Daniel R ,  LOMBERK, Scott Ellis ,  LOTVIN, Jason Arnold ,  PATEL-BROWN, Sujata Kaushikbhai ,  SUN, Weiqiang ,  TOMASELLO, Peter Anthony

IPC分类号： C12N1/20 ,  C12R1/46

CPC分类号： C12N1/20 ,  C12P19/04 ,  C12R1/46

摘要： The present invention relates to media and fermentation methods for producing polysaccharides in bacterial cell culture. In one aspect, the invention relates to a complex culture medium comprising a vegetable hydrolysate, a yeast extract, and a carbon source. In another aspect, the invention relates to a defined media having a total amino acid concentration greater than about 50 mM. A further aspect of the invention relates to the use of fed batch and perfusion fermentation methods for cultivating polysaccharide- producing bacteria.

公开(公告)号：EP2909307A2

公开(公告)日：2015-08-26

申请号：EP13786535.8

申请日：2013-10-07

申请人： Pfizer Inc.

发明人： JANSEN, Kathrin Ute ,  ANDERSON, Annaliesa Sybil ,  DONALD, Robert G.K. ,  FLINT, Michael James ,  KALYAN, Narender Kumar ,  LOTVIN, Jason Arnold ,  SIDHU, Maninder K ,  MORAN, Justin Keith ,  RUPPEN, Mark Edward ,  SUN, Weiqiang

IPC分类号： C12N1/20 ,  C07K14/33

CPC分类号： C12N9/1051 ,  C07K14/33 ,  C12N1/20

摘要： In one aspect, the invention relates to an immunogenic composition that includes a mutant
Clostridium difficile toxin A and/or a mutant
Clostridium difficile toxin B. The mutant toxin may include a glucosyltransferase domain having at least one mutation and a cysteine protease domain having at least one mutation, relative to the corresponding wild-type
C. difficile toxin. The mutant toxins may include at least one amino acid that is chemically crosslinked. In another aspect, the invention relates to methods and compositions for use in culturing Clostridium difficile and in producing
C. difficile toxins.

摘要翻译： 一方面，本发明涉及包含突变型艰难梭菌毒素A和/或突变型艰难梭菌毒素B的免疫原性组合物。突变毒素可以包括具有至少一个突变的葡糖基转移酶结构域和至少具有至少一个突变的半胱氨酸蛋白酶结构域 一个突变，相对于相应的野生型艰难梭菌毒素。 突变毒素可以包括至少一个化学交联的氨基酸。 另一方面，本发明涉及用于培养艰难梭菌和产生艰难梭菌毒素的方法和组合物。