METHOD FOR THE DETECTION OF MICROORGANISMS IN PHARMACEUTICAL PRODUCTS
    1.
    发明公开
    METHOD FOR THE DETECTION OF MICROORGANISMS IN PHARMACEUTICAL PRODUCTS 审中-公开
    方法MIKROORGASNISMEN的制药产品检测

    公开(公告)号:EP1565576A2

    公开(公告)日:2005-08-24

    申请号:EP03779871.7

    申请日:2003-11-07

    IPC分类号: C12Q1/68

    CPC分类号: C12Q1/04 C12Q1/689

    摘要: The present invention discloses a method for the detection and identification of living microbial contaminant cells in pharmaceutical products, pharmaceutical production environments, cosmetics and food. The method of the invention is based on the selective amplification by quantitative PCR of specific target cDNA sequences, in particular sequences encoding ribosomal RNA of the microbial contaminant to be detected.The invention also provides for a test kit for the detection and identification of living microbial contaminant cells in pharmaceutical products, pharmaceutical production environments, cosmetics and food comprising primers and probes specific for a ribosomal RNA target gene of the microbial contaminant.