公开(公告)号：EP1893638A2

公开(公告)日：2008-03-05

申请号：EP06772278.5

申请日：2006-06-06

申请人： Pioneer-Hi-Bred International, Inc. ,  E. I. DU PONT DE NEMOURS AND COMPANY

发明人： ABAD, Andre R. ,  CHUN, Gary C. ,  FLANNAGAN, Ronald D. ,  HERRMANN, Rafael ,  LU, Albert L. ,  MCCUTCHEN, Billy F. ,  RICE, Janet A. ,  SCHEPERS, Eric J.

IPC分类号： C07K14/325 ,  C12N9/64 ,  A01N63/02 ,  C12N15/82

CPC分类号： C12N15/8286 ,  A01N63/02 ,  C07K14/325 ,  C12N9/6472 ,  Y02A40/162

摘要： Compositions and methods for protecting a plant from an insect pest are provided. Nucleic acid molecules encoding insect protoxins or insect toxins modified to comprise at least one proteolytic activation site that is sensitive to an insect gut protease are provided. Cleavage of a modified insect protoxin at the proteolytic activation site by an insect gut protease produces an active insect toxin in the gut of the insect pest. Cleavage of a modified insect toxin of the invention at a proteolytic activation site results in the production of an active insect toxin in the insect gut that displays improved pesticidal activity relative to the insect toxin that lacks the proteolytic activation site. Methods of using the modified insect protoxin and modified insect toxin nucleic acid sequences and the polypeptides they encode to protect a plant from an insect pest are provided. Particular embodiments of the invention further provide modified insect protoxin and modified insect toxin compositions and formulations, expression cassettes, and transformed plants, plant cells, and seeds. Insect gut proteases and the nucleic acid molecules that encode them are also disclosed herein.

公开(公告)号：EP1893638B1

公开(公告)日：2011-08-10

申请号：EP06772278.5

申请日：2006-06-06

申请人： Pioneer-Hi-Bred International, Inc. ,  E.I. DU PONT DE NEMOURS AND COMPANY

发明人： ABAD, Andre R. ,  CHUN, Gary C. ,  FLANNAGAN, Ronald D. ,  HERRMANN, Rafael ,  LU, Albert L. ,  MCCUTCHEN, Billy F. ,  RICE, Janet A. ,  SCHEPERS, Eric J.

IPC分类号： C07K14/325 ,  C12N9/64 ,  A01N63/02 ,  C12N15/82

CPC分类号： C12N15/8286 ,  A01N63/02 ,  C07K14/325 ,  C12N9/6472 ,  Y02A40/162

摘要： Compositions and methods for protecting a plant from an insect pest are provided. Nucleic acid molecules encoding insect protoxins or insect toxins modified to comprise at least one proteolytic activation site that is sensitive to an insect gut protease are provided. Cleavage of a modified insect protoxin at the proteolytic activation site by an insect gut protease produces an active insect toxin in the gut of the insect pest. Cleavage of a modified insect toxin of the invention at a proteolytic activation site results in the production of an active insect toxin in the insect gut that displays improved pesticidal activity relative to the insect toxin that lacks the proteolytic activation site. Methods of using the modified insect protoxin and modified insect toxin nucleic acid sequences and the polypeptides they encode to protect a plant from an insect pest are provided. Particular embodiments of the invention further provide modified insect protoxin and modified insect toxin compositions and formulations, expression cassettes, and transformed plants, plant cells, and seeds. Insect gut proteases and the nucleic acid molecules that encode them are also disclosed herein.

公开(公告)号：EP1866419A2

公开(公告)日：2007-12-19

申请号：EP06749232.2

申请日：2006-04-04

申请人： Pioneer-Hi-Bred International, Inc. ,  E.I. DUPONT DE NEMOURS AND COMPANY

发明人： ABAD, Andre R. ,  FLANNAGAN, Ronald D. ,  HERRMANN, Rafael ,  LU, Albert L. ,  MCCUTCHEN, Billy F. ,  SIMMONS, Carl, R

IPC分类号： C12N15/82 ,  C12N15/67 ,  A01H5/00

CPC分类号： C12N15/8216 ,  C12N15/8286 ,  Y02A40/162

摘要： The present invention relates to methods of designing nucleic acid molecules for improved expression of the encoded polypeptides in plants. In such methods, codon usage frequencies are biased towards codon usage frequencies of a plant virus, group of plant viruses, or a subset of nucleic acid molecules therefrom. In preferred embodiments, the encoded polypeptide affects the phenotype of the plant. The invention also pertains to nucleic acid molecules encoding insecticidal polypeptides wherein the nucleic acid molecules have been designed to have plant virus codon-biased. The invention also pertains to transgenic plants and progeny thereof with increased expression of insecticidal polypeptides for improved resistance to insects and other pests that are detrimental to plants of agricultural value.