公开(公告)号：EP1866419A2

公开(公告)日：2007-12-19

申请号：EP06749232.2

申请日：2006-04-04

申请人： Pioneer-Hi-Bred International, Inc. ,  E.I. DUPONT DE NEMOURS AND COMPANY

发明人： ABAD, Andre R. ,  FLANNAGAN, Ronald D. ,  HERRMANN, Rafael ,  LU, Albert L. ,  MCCUTCHEN, Billy F. ,  SIMMONS, Carl, R

IPC分类号： C12N15/82 ,  C12N15/67 ,  A01H5/00

CPC分类号： C12N15/8216 ,  C12N15/8286 ,  Y02A40/162

摘要： The present invention relates to methods of designing nucleic acid molecules for improved expression of the encoded polypeptides in plants. In such methods, codon usage frequencies are biased towards codon usage frequencies of a plant virus, group of plant viruses, or a subset of nucleic acid molecules therefrom. In preferred embodiments, the encoded polypeptide affects the phenotype of the plant. The invention also pertains to nucleic acid molecules encoding insecticidal polypeptides wherein the nucleic acid molecules have been designed to have plant virus codon-biased. The invention also pertains to transgenic plants and progeny thereof with increased expression of insecticidal polypeptides for improved resistance to insects and other pests that are detrimental to plants of agricultural value.

公开(公告)号：EP1385370A2

公开(公告)日：2004-02-04

申请号：EP02726780.6

申请日：2002-04-19

申请人： PIONEER HI-BRED INTERNATIONAL, INC. ,  E.I. DUPONT DE NEMOURS AND COMPANY

发明人： ALTIER, Daniel, J. ,  HERRMANN, Rafael ,  LU, Albert, L. ,  McCUTCHEN, Billy, F. ,  PRESNAIL, James, K. ,  WEAVER, Janine, L. ,  WONG, James, F., H.

IPC分类号： A01H5/00 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/04 ,  C12N7/01 ,  C12N15/12 ,  C12N15/70 ,  C12N15/74 ,  C12N15/80 ,  C12N15/81 ,  C12N15/82 ,  C07K14/435

CPC分类号： C12N15/8282 ,  C07K14/43563 ,  Y10S530/858

摘要： The methods and compositions of the present invention find use in impacting microbial pathogens and in enhancing disease resistance to pathogens, particularly by plants. The compositions of the invention include polypeptides that possess antimicrobial properties, particularly fungicidal properties, and the encoding nucleic acid molecules. The polypeptides of the invention are isolated from the hemolymph and fat bodies of insect larvae induced by injection of plant pathogenic fungi. Further provided are plant cells, plants, an seed thereof, transformed with the nucleic acid mole cules of the invention so as to confer disease resistance on the plant.

公开(公告)号：EP2235182B1

公开(公告)日：2013-07-17

申请号：EP09702253.7

申请日：2009-01-15

申请人： Pioneer Hi-Bred International Inc. ,  E. I. du Pont de Nemours and Company

发明人： HERRMANN, Rafael ,  LASSNER, Michael ,  LU, Albert L. ,  NELSON, Mark ,  PRESNAIL, James K. ,  RICE, Janet A.

IPC分类号： C12N15/82

CPC分类号： C12N15/8286 ,  A01N65/20 ,  A01N65/44 ,  C12N15/113 ,  C12N2310/11 ,  C12N2310/14 ,  C12N2310/531 ,  C12N2320/32 ,  Y02A40/162

摘要： Methods and compositions are provided which employ a silencing element that, when ingested by a pest, such as a pest from the Lepidoptera order, they are capable of decreasing the expression of a target sequence in the pest. In specific embodiments, the decrease in expression of the target sequence controls the pest and thereby the methods and compositions are capable of limiting damage to a plant. The present invention provides target polynucleotides encoding polypeptides from specific protein families and various target polynucleotides set forth in SEQ ID NOS: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, or 50 or active variants and fragments thereof, wherein a decrease in expression of one or more the sequences in the target pest controls the pest (i.e., has insecticidal activity). Further provided are silencing elements which when ingested by the pest decrease the level of the target polypeptide and thereby control the pest. In specific embodiment, the pest is Spodoptera frugiperda. Plants, plant part, bacteria and other host cells comprising the silencing elements or an active variant or fragment thereof of the invention are also provided.

公开(公告)号：EP2235182A2

公开(公告)日：2010-10-06

申请号：EP09702253.7

申请日：2009-01-15

申请人： Pioneer Hi-Bred International Inc. ,  E. I. du Pont de Nemours and Company

发明人： HERRMANN, Rafael ,  LASSNER, Michael ,  LU, Albert L. ,  NELSON, Mark ,  PRESNAIL, James K. ,  RICE, Janet A.

IPC分类号： C12N15/82

CPC分类号： C12N15/8286 ,  A01N65/20 ,  A01N65/44 ,  C12N15/113 ,  C12N2310/11 ,  C12N2310/14 ,  C12N2310/531 ,  C12N2320/32 ,  Y02A40/162

摘要： Methods and compositions are provided which employ a silencing element that, when ingested by a pest, such as a pest from the Lepidoptera order, they are capable of decreasing the expression of a target sequence in the pest. In specific embodiments, the decrease in expression of the target sequence controls the pest and thereby the methods and compositions are capable of limiting damage to a plant. The present invention provides target polynucleotides encoding polypeptides from specific protein families and various target polynucleotides set forth in SEQ ID NOS: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, or 50 or active variants and fragments thereof, wherein a decrease in expression of one or more the sequences in the target pest controls the pest (i.e., has insecticidal activity). Further provided are silencing elements which when ingested by the pest decrease the level of the target polypeptide and thereby control the pest. In specific embodiment, the pest is Spodoptera frugiperda. Plants, plant part, bacteria and other host cells comprising the silencing elements or an active variant or fragment thereof of the invention are also provided.

公开(公告)号：EP1709068A2

公开(公告)日：2006-10-11

申请号：EP04814870.4

申请日：2004-12-20

申请人： PIONEER HI-BRED INTERNATIONAL, INC. ,  E.I. du Pont de Nemours and Company

发明人： ABAD, André ,  FLANNAGAN, Ronald, D. ,  HERRMANN, Rafael ,  LU, Albert ,  MCCUTCHEN, Billy, F. ,  PRESNAIL, James, K. ,  RICE, Janet, A. ,  WONG, James, F. ,  YU, Cao-Guo

IPC分类号： C07K14/325 ,  C12N9/64 ,  C12N15/82 ,  A01H5/02

CPC分类号： C12N15/8286 ,  C12N9/641 ,  Y02A40/162

摘要： Compositions and methods for protecting a plant from an insect pest are provided. In particular, nucleic acid sequences encoding insect protoxins modified to comprise at least one proteolytic activation site that is sensitive to a plant protease or an insect gut protease are provided. Cleavage of the modified protoxin at the proteolytic activation site by a protease produces an active insect toxin. Methods of using the modified insect protoxin nucleic acid sequences and the polypeptides they encode to protect a plant from an insect pest are provided. Particular embodiments of the invention further provide modified insect protoxin compositions and formulations, expression cassettes, and transformed plants, plant cells, and seeds.

公开(公告)号：EP1572880B1

公开(公告)日：2014-01-22

申请号：EP02756376.6

申请日：2002-06-21

申请人： PIONEER HI-BRED INTERNATIONAL, INC. ,  E.I. DU PONT DE NEMOURS AND COMPANY

发明人： CAHOON, Rebecca, E. ,  HERRMANN, Rafael ,  HARVELL, Leslie, T. ,  LU, Albert, Laurence ,  MCCUTCHEN, Billy, Fred ,  NAVARRO ACEVEDO, Pedro, A. ,  SIMMONS, Carl, R. ,  WONG, James, F., H.

IPC分类号： C12N15/29 ,  C12N15/82 ,  C07K14/415

CPC分类号： C07K14/4723 ,  C07K14/415 ,  C12N15/8279

公开(公告)号：EP1763536A1

公开(公告)日：2007-03-21

申请号：EP05788923.0

申请日：2005-07-01

申请人： Pioneer-Hi-Bred International, Inc. ,  E.I. du Pont de Nemours and Company ,  The Regents of the University of California

发明人： ALTIER, Daniel, J., Altier ,  DAHLBACKA, Glen ,  HERRMANN, Rafael ,  HUNTER-CEVERA, Jennie ,  McCUTCHEN, Billy, Fred ,  PRESNAIL, James, K. ,  SCHEPERS, Eric, J. ,  RICE, Janet, A. ,  SIMMONS, Carl, R. ,  TOROK, Tamas ,  YALPANI, Nasser ,  ELLESKAYA, I., A.

IPC分类号： C07K14/385 ,  C07K14/37

CPC分类号： C07K14/37 ,  A01N63/02 ,  A01N63/04 ,  A61K38/00 ,  C07K14/385 ,  C12N15/8282

摘要： Compositions and methods for protecting a plant from a pathogen, particularly a fungal pathogen, are provided. Compositions include novel amino acid sequences, and variants and fragments thereof, for antipathogenic polypeptides that were isolated from microbial fermentation broths. Nucleic acid molecules comprising nucleotide sequences that encode the antipathogenic polypeptides of the invention are also provided. A method for inducing pathogen resistance in a plant using the nucleotide sequences disclosed herein is further provided. The method comprises introducing into a plant an expression cassette comprising a promoter operably linked to a nucleotide sequence that encodes an antipathogenic polypeptide of the invention. Compositions comprising an antipathogenic polypeptide or a transformed microorganism comprising a nucleic acid of the invention in combination with a carrier and methods of using these compositions to protect a plant from a pathogen are further provided. Transformed plants, plant cells, seeds, and microorganisms comprising a nucleotide sequence that encodes an antipathogenic polypeptide of the invention, or variant or fragment thereof, are also disclosed.

公开(公告)号：EP1572880A3

公开(公告)日：2005-12-07

申请号：EP02756376.6

申请日：2002-06-21

申请人： PIONEER HI-BRED INTERNATIONAL, INC. ,  E.I. DU PONT DE NEMOURS AND COMPANY

发明人： CAHOON, Rebecca, E. ,  HERRMANN, Rafael ,  HARVELL, Leslie, T. ,  LU, Albert, Laurence ,  MCCUTCHEN, Billy, Fred ,  NAVARRO ACEVEDO, Pedro, A. ,  SIMMONS, Carl, R. ,  WONG, James, F., H.

IPC分类号： C12N1/00

公开(公告)号：EP2281049A2

公开(公告)日：2011-02-09

申请号：EP09701991.3

申请日：2009-01-15

申请人： Pioneer Hi-Bred International Inc. ,  E. I. du Pont de Nemours and Company

发明人： HERRMANN, Rafael ,  LASSNER, Michael ,  LU, Albert, L. ,  NELSON, Mark ,  PRESNAIL, James, K. ,  RICE, Janet, A.

IPC分类号： C12N15/82

CPC分类号： C12N15/8286 ,  C07K14/43563 ,  Y02A40/162

摘要： Methods and compositions are provided which employ a silencing element that, when ingested by a pest, such as a pest from the Aphididae family, they are capable of decreasing the expression of a target sequence in the pest. In specific embodiments, the decrease in expression of the target sequence controls the pest and thereby the methods and compositions are capable of limiting damage to a plant. The present invention provides target polynucleotides for specific protein classes and also target polynucleotides as set forth in SEQ ID NOS:1, 2, 3, 4, 5, 6, 7, 8, 9, 10 or active variants or fragments thereof, wherein a decrease in expression of one or more the sequences in the target pest controls the pest (i.e., has insecticidal activity). Further provided are silencing elements which when ingested by the pest decrease the level of the target polypeptide and thereby control the pest. In specific embodiment, the pest is Aphis glycines. Plants, plant part, bacteria and other host cells comprising the silencing elements or an active variant or fragment thereof of the invention are also provided.

公开(公告)号：EP1763536B1

公开(公告)日：2010-09-08

申请号：EP05788923.0

申请日：2005-07-01

申请人： Pioneer-Hi-Bred International, Inc. ,  E. I. du Pont de Nemours and Company ,  The Regents of The University of California

发明人： ALTIER, Daniel, J. ,  DAHLBACKA, Glen ,  HERRMANN, Rafael ,  HUNTER-CEVERA, Jennie ,  McCUTCHEN, Billy, Fred ,  PRESNAIL, James, K. ,  SCHEPERS, Eric, J. ,  RICE, Janet, A. ,  SIMMONS, Carl, R. ,  TOROK, Tamas ,  YALPANI, Nasser ,  ELLESKAYA, I., A.

IPC分类号： C07K14/385 ,  C07K14/37

CPC分类号： C07K14/37 ,  A01N63/02 ,  A01N63/04 ,  A61K38/00 ,  C07K14/385 ,  C12N15/8282

摘要： Compositions and methods for protecting a plant from a pathogen, particularly a fungal pathogen, are provided. Compositions include novel amino acid sequences, and variants and fragments thereof, for antipathogenic polypeptides that were isolated from microbial fermentation broths. Nucleic acid molecules comprising nucleotide sequences that encode the antipathogenic polypeptides of the invention are also provided. A method for inducing pathogen resistance in a plant using the nucleotide sequences disclosed herein is further provided. The method comprises introducing into a plant an expression cassette comprising a promoter operably linked to a nucleotide sequence that encodes an antipathogenic polypeptide of the invention. Compositions comprising an antipathogenic polypeptide or a transformed microorganism comprising a nucleic acid of the invention in combination with a carrier and methods of using these compositions to protect a plant from a pathogen are further provided. Transformed plants, plant cells, seeds, and microorganisms comprising a nucleotide sequence that encodes an antipathogenic polypeptide of the invention, or variant or fragment thereof, are also disclosed.