公开(公告)号：EP0938506A1

公开(公告)日：1999-09-01

申请号：EP97934467.0

申请日：1997-07-16

申请人： MorphoSys AG ,  Plückthun, Andreas, Prof. Dr.

发明人： PLÜCKTHUN, Andreas ,  NIEBA, Lars ,  HONEGGER, Annemarie

IPC分类号： G01N33 ,  A61K38 ,  A61K39 ,  C07K16 ,  C12N1 ,  C12N5 ,  C12N15

CPC分类号： C07K16/00 ,  C07K16/44 ,  C07K2319/00

摘要： The present invention relates to the modification of immunoglobulin superfamily (IgSF) domains, IgSF fragments and fusion proteins thereof, especially to the modification of antibody derivatives, so as to improve their solubility, and hence the yield, and ease of handling. The inventors have found that this can be achieved by making the region which comprises the interface with domains adjoined to said IgSF domain in a larger fragment or a full IgSF protein, and which becomes exposed in the IgSF domain, more hydrophilic by modification. The present invention describes DNA sequences encoding modified IgSF domains or fragments and fusion proteins thereof, vectors and hosts containing these DNA sequences, IgSF domains or fragments or fusion proteins obtainable by expressing said DNA sequences in suitable expression systems, and a method for modifying IgSF domains, so as to improve their solubility, expressibility and ease of handling.

公开(公告)号：EP0938506B1

公开(公告)日：2003-11-05

申请号：EP97934467.8

申请日：1997-07-16

申请人： Plückthun, Andreas, Prof. Dr.

发明人： PLÜCKTHUN, Andreas ,  NIEBA, Lars ,  HONEGGER, Annemarie

IPC分类号： C07K16/00 ,  C12N15/12 ,  C12N15/13 ,  C12N15/63 ,  G01N33/53 ,  A61K38/17 ,  A61K39/395

CPC分类号： C07K16/00 ,  C07K16/44 ,  C07K2319/00

摘要： The present invention relates to the modification of immunoglobulin superfamily (IgSF) domains, IgSF fragments and fusion proteins thereof, especially to the modification of antibody derivatives, so as to improve their solubility, and hence the yield, and ease of handling. The inventors have found that this can be achieved by making the region which comprises the interface with domains adjoined to said IgSF domain in a larger fragment or a full IgSF protein, and which becomes exposed in the IgSF domain, more hydrophilic by modification. The present invention describes DNA sequences encoding modified IgSF domains or fragments and fusion proteins thereof, vectors and hosts containing these DNA sequences, IgSF domains or fragments or fusion proteins obtainable by expressing said DNA sequences in suitable expression systems, and a method for modifying IgSF domains, so as to improve their solubility, expressibility and ease of handling.