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    CONTROL OF EMULSIONS, INCLUDING MULTIPLE EMULSIONS
    1.
    发明公开
    CONTROL OF EMULSIONS, INCLUDING MULTIPLE EMULSIONS 有权
    芫荽粉，茉莉酸

    公开(公告)号：EP2714254A2

    公开(公告)日：2014-04-09

    申请号：EP12725967.9

    申请日：2012-05-22

    申请人： President and Fellows of Harvard College ,  BASF SE

    发明人： ROTEM, Assaf ,  WEITZ, David A. ,  ABATE, Adam R. ,  HOLTZE, Christian

    IPC分类号： B01F13/00 ,  B01F3/08

    CPC分类号： B01F3/0811 ,  B01F3/0807 ,  B01F13/0062 ,  B01F13/0084 ,  Y10T137/87571

    摘要： The present invention generally relates to emulsions, and more particularly, to double and other multiple emulsions. Certain aspects of the present invention are generally directed to the creation of double emulsions and other multiple emulsions at a common junction of microfluidic channels. In some cases, the microfluidic channels at the common junction may have substantially the same hydrophobicity. In one set of embodiments, a device may include a common junction of six or more channels, where a first fluid flows through one channel, a second fluid flows through two channels, and a third or carrying fluid flows through two more channels, such that a double emulsion of a first droplet of the first fluid, contained in a second droplet of the second fluid, contained by the carrying fluid, flows away from the common junction through a sixth channel. Other aspects of the invention are generally directed to methods of making and using such systems, kits involving such systems, emulsions created using such systems, or the like.

    摘要翻译： 本发明一般涉及乳液，更具体地说涉及双重和其它多重乳液。 本发明的某些方面通常涉及在微流体通道的共同连接处产生双重乳液和其它多重乳液。 在一些情况下，共同连接处的微流体通道可具有基本上相同的疏水性。 在一组实施例中，装置可以包括六个或更多个通道的公共接头，其中第一流体流过一个通道，第二流体流过两个通道，并且第三或承载流体流过两个以上通道，使得 包含在第二流体的第二液滴中的第一流体的第一液滴的双重乳液由携带流体包含，通过第六通道从公共接头流出。

    SYSTEMS AND METHODS FOR EPIGENETIC SEQUENCING
    4.
    发明公开
    SYSTEMS AND METHODS FOR EPIGENETIC SEQUENCING 审中-公开
    用于表观测序的系统和方法

    公开(公告)号：EP2823064A1

    公开(公告)日：2015-01-14

    申请号：EP13758373.8

    申请日：2013-03-05

    申请人： President and Fellows of Harvard College ,  The General Hospital Corporation d/b/a Massachusetts General Hospital

    发明人： ROTEM, Assaf ,  RAM, Oren ,  BERNSTEIN, Bradley, E. ,  WEITZ, David, A.

    IPC分类号： C12Q1/68 ,  C12Q1/34 ,  C12Q1/44 ,  C07H21/00

    CPC分类号： C12Q1/6869 ,  B01F13/0071 ,  B01L3/502784 ,  C12Q2521/301 ,  C12Q2523/301 ,  C12Q2525/131 ,  C12Q2525/161 ,  C12Q2563/179 ,  C12Q2565/629 ,  C12Q2563/159

    摘要： The present invention generally relates to microfluidics and epigenetic sequencing. In one set of embodiments, cells contained within a plurality of microfluidic droplets are lysed and the DNA (e.g., from nucleosomes) within the droplets are labeled, e.g., with adapters containing an identification sequence. The adapters may also contain other sequences, e.g., restriction sites, primer sites, etc., to assist with later analysis. After labeling with adapters, the DNA from the different cells may be combined and analyzed, e.g., to determine epigenetic information about the cells. For example, the DNA may be separated on the basis of certain modifications (e.g., methylation), and the DNA from the separated nucleosomes may be sequenced using techniques such as chromatin immunoprecipitation ("ChIP"). In some cases, the DNA sequences may also be aligned with genomes, e. g., to determine which portions of the genome were epigenetically modified, e. g., via methylation.

    摘要翻译： 本发明一般涉及微流体和表观遗传测序。 在一组实施方案中，包含在多个微流体液滴内的细胞被裂解，并且液滴内的DNA（例如来自核小体）被标记，例如用含有识别序列的衔接子进行标记。 衔接子还可以包含其他序列，例如限制性位点，引物位点等，以辅助以后的分析。 在用衔接子标记后，来自不同细胞的DNA可以被组合并分析，例如以确定关于细胞的表观遗传信息。 例如，可以基于某些修饰（例如甲基化）分离DNA，并且可以使用诸如染色质免疫沉淀（“ChIP”）的技术对来自分离的核小体的DNA进行测序。 在一些情况下，也可将DNA序列与基因组进行比对，例如以确定基因组的哪些部分经过表观遗传修饰，例如经由甲基化。