公开(公告)号：EP2714254A2

公开(公告)日：2014-04-09

申请号：EP12725967.9

申请日：2012-05-22

申请人： President and Fellows of Harvard College ,  BASF SE

发明人： ROTEM, Assaf ,  WEITZ, David A. ,  ABATE, Adam R. ,  HOLTZE, Christian

IPC分类号： B01F13/00 ,  B01F3/08

CPC分类号： B01F3/0811 ,  B01F3/0807 ,  B01F13/0062 ,  B01F13/0084 ,  Y10T137/87571

摘要： The present invention generally relates to emulsions, and more particularly, to double and other multiple emulsions. Certain aspects of the present invention are generally directed to the creation of double emulsions and other multiple emulsions at a common junction of microfluidic channels. In some cases, the microfluidic channels at the common junction may have substantially the same hydrophobicity. In one set of embodiments, a device may include a common junction of six or more channels, where a first fluid flows through one channel, a second fluid flows through two channels, and a third or carrying fluid flows through two more channels, such that a double emulsion of a first droplet of the first fluid, contained in a second droplet of the second fluid, contained by the carrying fluid, flows away from the common junction through a sixth channel. Other aspects of the invention are generally directed to methods of making and using such systems, kits involving such systems, emulsions created using such systems, or the like.

摘要翻译： 本发明一般涉及乳液，更具体地说涉及双重和其它多重乳液。 本发明的某些方面通常涉及在微流体通道的共同连接处产生双重乳液和其它多重乳液。 在一些情况下，共同连接处的微流体通道可具有基本上相同的疏水性。 在一组实施例中，装置可以包括六个或更多个通道的公共接头，其中第一流体流过一个通道，第二流体流过两个通道，并且第三或承载流体流过两个以上通道，使得 包含在第二流体的第二液滴中的第一流体的第一液滴的双重乳液由携带流体包含，通过第六通道从公共接头流出。

公开(公告)号：EP2714254B1

公开(公告)日：2017-09-06

申请号：EP12725967.9

申请日：2012-05-22

申请人： President and Fellows of Harvard College ,  BASF SE

发明人： ROTEM, Assaf ,  WEITZ, David A. ,  ABATE, Adam R. ,  HOLTZE, Christian

IPC分类号： B01F13/00 ,  B01F3/08

CPC分类号： B01F3/0811 ,  B01F3/0807 ,  B01F13/0062 ,  B01F13/0084 ,  Y10T137/87571

公开(公告)号：EP4019977A1

公开(公告)日：2022-06-29

申请号：EP21208171.5

申请日：2010-06-25

申请人： President and Fellows of Harvard College

发明人： WEITZ, David A. ,  ABATE, Adam R. ,  HUNG, Tony ,  MARY, Pascaline

IPC分类号： G01N35/10 ,  B01J19/00 ,  B01F25/314 ,  B01F33/3031

摘要： Systems (200) and methods for the control of fluids and, in some cases, to systems and methods for flowing a fluid into and/or out of other fluids. As examples, fluid may be injected into a droplet (220) contained within a fluidic channel (230), or a fluid may be injected into a fluidic channel to create a droplet (220). In some embodiments, electrodes (250) may be used to apply an electric field to one or more fluidic channels (230,240), e.g., proximate an intersection of at least two fluidic channels (230, 240). For instance, a first fluid may be urged into and/or out of a second fluid, facilitated by the electric field. The electric field, in some cases, may disrupt an interface between a first fluid and at least one other fluid. Properties such as the volume, flow rate, etc. of a first fluid being urged into and/or out of a second fluid can be controlled by controlling various properties of the fluid and/or a fluidic droplet, for example curvature of the fluidic droplet, and/or controlling the applied electric field.

公开(公告)号：EP3150724A1

公开(公告)日：2017-04-05

申请号：EP16197694.9

申请日：2009-12-18

申请人： President and Fellows of Harvard College

发明人： WEITZ, David A. ,  ABATE, Adam R.

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6809 ,  B01L3/502761 ,  B01L2200/10 ,  C12Q1/6834 ,  C12Q1/6869 ,  C12Q1/6874 ,  C12Q2525/204 ,  C12Q2563/149 ,  C12Q2563/159 ,  C12Q2565/629

摘要： This invention generally relates to particle-assisted nucleic acid sequencing. In some embodiments, sequencing may be performed in a microfluidic device, which can offer desirable properties, for example, minimal use of reagents, facile scale-up, and/or high throughput. In one embodiment, a target nucleic acid may be exposed to particles having nucleic acid probes. By determining the binding of the particles to the target nucleic acid, the sequence of the target nucleic acid (or at least a portion of the target nucleic acid) can be determined. The target nucleic acid may be encapsulated within a fluidic droplet with the particles having nucleic acid probes, in certain instances. In some cases, the sequence of the target nucleic acid may be determined, based on binding of the particles, using sequencing by hybridization (SBH) algorithms or other known techniques.

摘要翻译： 本发明一般涉及颗粒辅助核酸测序。 在一些实施方案中，可以在微流体装置中进行测序，所述微流体装置可以提供期望的性质，例如最小使用试剂，容易的放大和/或高通量。 在一个实施方案中，靶核酸可以暴露于具有核酸探针的颗粒。 通过确定颗粒与靶核酸的结合，可以确定靶核酸（或目标核酸的至少一部分）的序列。 在某些情况下，靶核酸可以包封在具有核酸探针的颗粒的流体液滴内。 在一些情况下，可以通过使用通过杂交（SBH）算法或其他已知技术进行测序，基于颗粒的结合来确定靶核酸的序列。