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公开(公告)号：EP0834758A2
公开(公告)日：1998-04-08
申请号：EP97120924.2
申请日：1995-06-30
申请人： Price, Jeffrey H.
发明人： Price, Jeffrey H.
CPC分类号： G02B21/244 , G01N15/147 , G01N21/6458 , G02B21/002
摘要： An apparatus for generating a volume image, for incorporation in an autofocus system for scanning microscopy is provided, including a plurality of imaging devices (436,481) which are coupled to an image forming apparatus (437, 440, 481) and used to form a plurality of electronic image representations. The storage (443) and processing (442)of successive pluralities of electronic image representations, wherein the plurality of electronic image representations represent images at respective image planes of the plurality of image planes, allows one to acquire a volume image.
摘要翻译： 提供了一种用于产生体积图像的装置，用于结合在用于扫描显微术的自动对焦系统中，包括耦合到图像形成装置（437,440,481）并用于形成多个的多个成像装置（436,481） 的电子图像表示。 连续多个电子图像表示的存储（443）和处理（442），其中所述多个电子图像表示表示在所述多个图像平面中的各个图像平面处的图像，允许获取体积图像。
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公开(公告)号：EP0834758B1
公开(公告)日：1999-09-01
申请号：EP97120924.2
申请日：1995-06-30
申请人： Price, Jeffrey H.
发明人： Price, Jeffrey H.
CPC分类号： G02B21/244 , G01N15/147 , G01N21/6458 , G02B21/002
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公开(公告)号：EP0769159B1
公开(公告)日：1999-03-10
申请号：EP95925491.3
申请日：1995-06-30
申请人： Price, Jeffrey H.
发明人： Price, Jeffrey H.
CPC分类号： G02B21/244 , G01N15/147 , G01N21/6458 , G02B21/002
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公开(公告)号：EP0834758A3
公开(公告)日：1998-05-06
申请号：EP97120924.2
申请日：1995-06-30
申请人： Price, Jeffrey H.
发明人： Price, Jeffrey H.
CPC分类号： G02B21/244 , G01N15/147 , G01N21/6458 , G02B21/002
摘要： An apparatus for generating a volume image, for incorporation in an autofocus system for scanning microscopy is provided, including a plurality of imaging devices (436,481) which are coupled to an image forming apparatus (437, 440, 481) and used to form a plurality of electronic image representations. The storage (443) and processing (442)of successive pluralities of electronic image representations, wherein the plurality of electronic image representations represent images at respective image planes of the plurality of image planes, allows one to acquire a volume image.
摘要翻译： 提供了一种用于产生体积图像的设备，用于并入用于扫描显微镜的自动聚焦系统中，包括多个成像设备（436,481），其耦合到图像形成设备（437,440,481）并用于形成多个 的电子图像表示。 连续多个电子图像表示的存储（443）和处理（442）允许人们获取体积图像，其中多个电子图像表示表示多个图像平面中的各个图像平面处的图像。
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公开(公告)号：EP0769159A1
公开(公告)日：1997-04-23
申请号：EP95925491.0
申请日：1995-06-30
申请人： Price, Jeffrey H.
发明人： Price, Jeffrey H.
CPC分类号： G02B21/244 , G01N15/147 , G01N21/6458 , G02B21/002
摘要： Reliable autofocus is required to obtain accurate measurements of fluorescent stained cellular components from a system capable of scanning multiple microscope fields. Autofocus could be performed directly with fluorescence images, but due to photobleaching and destructive fluorescence by-products, it is best to minimize fluorescence exposure for photosensitive specimens and live cells. This exposure problem could be completely avoided by using phase-contrast microscopy, implemented through the same optics as fluorescence microscopy. Functions for both phase-contrast and fluorescence autofocus were evaluated using the present invention and the suitability of phase-contrast autofocus for fluorescence microscopy was determined. The present autofocus system for scanning microscopy can be performed at least as fast as 0.25 s/field without loss of precision. The speed of autofocus can be further increased by a volume image which is obtained by observing an image object at each image plane of a plurality of image planes, where each image plane is vertically displaced with respect to each other image plane. An electronic image representation is obtained at each image plane. The image planes are scanned over the image object and the images are aligned timewise in a buffer. The buffer holds a volume image comprising images at the image planes, aligned by the buffer. The image plane having the best focus is selected and a microscope objective is automatically positioned at the selected plane.
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