公开(公告)号：EP4219745A3

公开(公告)日：2023-12-06

申请号：EP23156583.9

申请日：2014-06-25

申请人： Prognosys Biosciences, Inc.

发明人： Chee, Mark S. ,  Routenberg, David

IPC分类号： C12Q1/6841 ,  C12Q1/6853

摘要： The present disclosure provides methods and assay systems for use in spatially encoded biological assays, including assays to determine a spatial pattern of abundance, expression, and/or activity of one or more biological targets across multiple sites in a sample. In particular, the present disclosure provides methods and assay systems capable of high levels of multiplexing where reagents are provided to a biological sample in order to address tag the sites to which reagents are delivered; instrumentation capable of controlled delivery of reagents, in particular, microfluidic device based instrumentation; and a decoding scheme providing a readout that is digital in nature.

公开(公告)号：EP4219745A2

公开(公告)日：2023-08-02

申请号：EP23156583.9

申请日：2014-06-25

申请人： Prognosys Biosciences, Inc.

发明人： Chee, Mark S. ,  Routenberg, David

IPC分类号： C12Q1/68

摘要： The present disclosure provides methods and assay systems for use in spatially encoded biological assays, including assays to determine a spatial pattern of abundance, expression, and/or activity of one or more biological targets across multiple sites in a sample. In particular, the present disclosure provides methods and assay systems capable of high levels of multiplexing where reagents are provided to a biological sample in order to address tag the sites to which reagents are delivered; instrumentation capable of controlled delivery of reagents, in particular, microfluidic device based instrumentation; and a decoding scheme providing a readout that is digital in nature.

公开(公告)号：EP4234716A3

公开(公告)日：2023-12-06

申请号：EP23162693.8

申请日：2014-06-25

申请人： Prognosys Biosciences, Inc.

发明人： Chee, Mark S. ,  Routenberg, David

IPC分类号： C12Q1/6841 ,  C12Q1/6853

摘要： The present disclosure provides methods and assay systems for use in spatially encoded biological assays, including assays to determine a spatial pattern of abundance, expression, and/or activity of one or more biological targets across multiple sites in a sample. In particular, the biological targets comprise proteins, and the methods and assay systems do not depend on imaging techniques for the spatial information of the targets. The present disclosure provides methods and assay systems capable of high levels of multiplexing where reagents are provided to a biological sample in order to address tag the sites to which reagents are delivered; instrumentation capable of controlled delivery of reagents; and a decoding scheme providing a readout that is digital in nature.

公开(公告)号：EP4234716A2

公开(公告)日：2023-08-30

申请号：EP23162693.8

申请日：2014-06-25

申请人： Prognosys Biosciences, Inc.

发明人： Chee, Mark S. ,  Routenberg, David

IPC分类号： C12Q1/6853 ,  C12Q1/6841

摘要： The present disclosure provides methods and assay systems for use in spatially encoded biological assays, including assays to determine a spatial pattern of abundance, expression, and/or activity of one or more biological targets across multiple sites in a sample. In particular, the biological targets comprise proteins, and the methods and assay systems do not depend on imaging techniques for the spatial information of the targets. The present disclosure provides methods and assay systems capable of high levels of multiplexing where reagents are provided to a biological sample in order to address tag the sites to which reagents are delivered; instrumentation capable of controlled delivery of reagents; and a decoding scheme providing a readout that is digital in nature.