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    Colorimetric immunoassay on solid surface, dipstick therefor and its preparation
    2.
    发明公开
    Colorimetric immunoassay on solid surface, dipstick therefor and its preparation 失效
    比色法免疫在坚固的表面，并用于测试棒及其制备方法。

    公开(公告)号：EP0125118A2

    公开(公告)日：1984-11-14

    申请号：EP84303021.4

    申请日：1984-05-04

    申请人： QUIDEL CORPORATION

    发明人： Wada, Henry Garrett ,  Baxter, Sarah R. ,  Rundle, Douglas D. ,  Kasper, Kenneth C. ,  Lankford, John C.

    IPC分类号： G01N33/52 ,  G01N33/74 ,  G01N33/76 ,  G01N33/535 ,  G01N33/543

    CPC分类号： G01N33/54306 ,  G01N33/54366 ,  G01N33/74

    摘要： @ The inventive concept provides an immunoassay using an enzyme label, in which the detection of the unknown substance is performed colorimetrically directly upon a solid surface. Preferably, the surface is an impermeable surface of a dipstick. To detect antigen in a biologically derived fluid sample, a dipstick containing bound antibody may be contacted with the sample and with a soluble enzyme-labeled antibody, to cause the antigen to bind to both the immobilized antibody and the labeled antibody on the surface. After separation of unreacted reagents, the solid surface is contacted with a substrate which converts from a soluble form to an insoluble colored form in the presence of the enzyme and deposits upon the surface of the solid surface. The solid surface is separated from the solution. The sandwich immunoassay technique may also be employed to detect antibody in the sample by reversing the roles of the antigen and antibody. The system is also applicable to a competitive binding technique. The invention further includes a colored dipstick produced by this means.

    摘要翻译： 本发明构思提供关于使用的酶标记免疫测定法，其中，所述未知物质的检测是在固体表面上进行比色直接。 优选地，所述表面是试纸的不可渗透的表面。 为了检测生物来源的液体样品中的抗原，含结合的抗体浸渍片可与样品和与可溶性酶标记抗体接触，以使抗原结合于这两种固定化抗体和所述表面上的标记的抗体。 未反应的试剂的分离后，将固体表面是与从可溶形式向不溶性着色以形成所述酶和沉积物在所述固体表面的表面存在一个转换基板接触。 固体表面被从溶液中分离。 因此，可以采用夹心免疫测定技术通过反转抗原和抗体的作用，以检测抗体于样品中。 该系统非常适用于竞争性结合的技术。 本发明还包括由该方法产生的有色试纸。