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公开(公告)号：EP1920047B1
公开(公告)日：2012-08-15
申请号：EP06792998.4
申请日：2006-08-24
申请人： Qiagen GmbH
CPC分类号： C12Q1/6813 , C08F8/30 , C08F8/40 , C12N5/0081 , C12Q1/6806 , C12Q1/6844 , C08F222/06
摘要： The invention relates to a method for separating living cells from a mixture of dead and living cells, which can optionally also contain cell fragments.
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公开(公告)号：EP2240582B1
公开(公告)日：2013-10-23
申请号：EP08866104.6
申请日：2008-12-29
申请人： Qiagen GmbH
发明人： HAHN, Peter , DENNIG, Jörg , BIELKE, Wolfgang , KANG, Jie
IPC分类号： C12N15/11 , C12Q1/68 , A61K31/713
CPC分类号： C12N15/1137 , C12N15/111 , C12N15/113 , C12N15/65 , C12N2310/111 , C12N2310/14 , C12N2310/53 , C12N2320/10
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公开(公告)号：EP1920047A1
公开(公告)日：2008-05-14
申请号：EP06792998.4
申请日：2006-08-24
申请人： Qiagen GmbH
CPC分类号： C12Q1/6813 , C08F8/30 , C08F8/40 , C12N5/0081 , C12Q1/6806 , C12Q1/6844 , C08F222/06
摘要： The invention relates to a method for separating living cells from a mixture of dead and living cells, which can optionally also contain cell fragments.
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公开(公告)号：EP2240582A1
公开(公告)日：2010-10-20
申请号：EP08866104.6
申请日：2008-12-29
申请人： Qiagen GmbH
发明人： HAHN, Peter , DENNIG, Jörg , BIELKE, Wolfgang , KANG, Jie
IPC分类号： C12N15/11 , C12Q1/68 , A61K31/713
CPC分类号： C12N15/1137 , C12N15/111 , C12N15/113 , C12N15/65 , C12N2310/111 , C12N2310/14 , C12N2310/53 , C12N2320/10
摘要： The invention pertains to the use of an apoptosis inducing combination of at least a. a first expression modulating compound silencing the expression of at least a first target gene involved in apoptosis and b. a second expression modulating compound silencing the expression of at least a second target gene involved in apoptosis as a positive control in expression modulating assays. Also provided are suitable methods, kits and compositions.
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公开(公告)号：EP1274462B1
公开(公告)日：2008-11-19
申请号：EP01936172.4
申请日：2001-04-03
申请人： QIAGEN GmbH
发明人： WEBER, Martin , DENNIG, Jörg , ERBACHER, Christoph
CPC分类号： B82Y5/00 , A61K47/645 , A61K47/6898 , C08G83/004 , C12N15/87
摘要： The invention relates to a method for carrying out the targeted transfection of cells, to compositions, which can be used for such methods, and to corresponding medicaments for use in gene therapy. The invention particularly relates to a method for introducing nucleic acid into cells involving the following steps: (a) mixing a nucleic acid with a dendrimer, whereby a portion of the dendrimer molecules is biotinylated; mixing the prepared complex, which consists of a nucleic acid and a dendrimer, with a second complex, which consists of an avidin or a streptavidin and of a biotinylated target-specific binding molecule, and; (c) incubating the complex prepared in step (b) with cells. Dendrimers that are well-suited for the invention are, for example, partially solvolyzed polyamidoamine (PAMAM) dendrimers. Target-specific binding molecules are, in particular, cell type-specific markers also of the cell surface of the target cells.
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公开(公告)号：EP1274462A2
公开(公告)日：2003-01-15
申请号：EP01936172.4
申请日：2001-04-03
申请人： QIAGEN GmbH
发明人： WEBER, Martin , DENNIG, Jörg , ERBACHER, Christoph
CPC分类号： B82Y5/00 , A61K47/645 , A61K47/6898 , C08G83/004 , C12N15/87
摘要： The invention relates to a method for carrying out the targeted transfection of cells, to compositions, which can be used for such methods, and to corresponding medicaments for use in gene therapy. The invention particularly relates to a method for introducing nucleic acid into cells involving the following steps: (a) mixing a nucleic acid with a dendrimer, whereby a portion of the dendrimer molecules is biotinylated; mixing the prepared complex, which consists of a nucleic acid and a dendrimer, with a second complex, which consists of an avidin or a streptavidin and of a biotinylated target-specific binding molecule, and; (c) incubating the complex prepared in step (b) with cells. Dendrimers that are well-suited for the invention are, for example, partially solvolyzed polyamidoamine (PAMAM) dendrimers. Target-specific binding molecules are, in particular, cell type-specific markers also of the cell surface of the target cells.
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