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29 条记录 (耗时 0.013 秒)

    RAPID METHOD FOR ISOLATING EXTRACELLULAR NUCLEIC ACIDS
    1.
    发明公开
    RAPID METHOD FOR ISOLATING EXTRACELLULAR NUCLEIC ACIDS 审中-公开

    公开(公告)号：EP4159857A1

    公开(公告)日：2023-04-05

    申请号：EP22206649.0

    申请日：2012-09-25

    申请人： QIAGEN GmbH

    发明人： HORLITZ, Martin Dr. ,  SPRENGER-HAUSSELS, Markus Dr. ,  GRÜNEFELD, Peter ,  NOCON, Annette ,  ERBACHER, Christoph

    IPC分类号： C12N15/10

    摘要： The present invention pertains to a method for isolating extracellular nucleic acids from a sample, wherein said sample is optionally stabilized, by binding the extracellular nucleic acids to a solid phase which carries anion exchange groups, comprising the following steps:
    a. binding the extracellular nucleic acids to the solid phase in a binding mixture having a first pH which allows binding the extracellular nucleic acids to the anion exchange groups of the solid phase;
    b. separating the solid phase with the bound extracellular nucleic acids;
    c. optionally washing the extracellular nucleic acids;
    d. optionally eluting extracellular nucleic acids from the solid phase.
    The method has the advantage that large sample volumes can be processed and that extracellular nucleic acids can be isolated rapidly with a high yield. The method is particularly suitable for automatable processes.

    CATIONIC REAGENTS FOR TRANSFECTION
    6.
    发明授权
    CATIONIC REAGENTS FOR TRANSFECTION 失效
    阳离子试剂避免染

    公开(公告)号：EP1003556B1

    公开(公告)日：2006-03-01

    申请号：EP97950064.2

    申请日：1997-11-03

    申请人： QIAGEN GmbH

    发明人： ERBACHER, Christoph ,  WEBER, Martin

    IPC分类号： A61K47/48

    CPC分类号： C12N15/88 ,  A61K9/1272 ,  A61K47/54 ,  A61K47/543 ,  A61K47/544 ,  A61K48/00

    摘要： The present invention relates to cationic cytofectins and liposomes comprising the same for use in delivering exogenous compounds into cells in vitro and in vivo. The liposome may comprise: (a) a neutral lipid such as dioleoylphosphatidylethanolamine (DOPE) or similar lipid-like compounds such as 1,2-dioleoyloxiphosphatidylethanolamine or other lipid-like structures and (b) one or more of the cationic cytofectins provided herein. The present invention also provides transfection kits and methods of delivery comprising the same.

    CATIONIC REAGENTS FOR TRANSFECTION
    7.
    发明公开
    CATIONIC REAGENTS FOR TRANSFECTION 失效
    阳离子试剂避免染

    公开(公告)号：EP1003556A2

    公开(公告)日：2000-05-31

    申请号：EP97950064.2

    申请日：1997-11-03

    申请人： QIAGEN GmbH

    发明人： ERBACHER, Christoph ,  WEBER, Martin

    IPC分类号： A61K47/48

    CPC分类号： C12N15/88 ,  A61K9/1272 ,  A61K47/54 ,  A61K47/543 ,  A61K47/544 ,  A61K48/00

    摘要： The present invention relates to cationic cytofectins and liposomes comprising the same for use in delivering exogenous compounds into cells in vitro and in vivo. The liposome may comprise: (a) a neutral lipid such as dioleoylphosphatidylethanolamine (DOPE) or similar lipid-like compounds such as 1,2-dioleoyloxiphosphatidylethanolamine or other lipid-like structures and (b) one or more of the cationic cytofectins provided herein. The present invention also provides transfection kits and methods of delivery comprising the same.

    METHODS AND KIT FOR ISOLATING NUCLEIC ACIDS
    10.
    发明公开
    METHODS AND KIT FOR ISOLATING NUCLEIC ACIDS 有权
    方法和试剂盒用于分离核酸

    公开(公告)号：EP2082039A1

    公开(公告)日：2009-07-29

    申请号：EP07818897.6

    申请日：2007-10-10

    申请人： Qiagen GmbH

    发明人： RITT, Christoph ,  ERBACHER, Christoph ,  BAUMHOF, Patrick

    IPC分类号： C12N15/10

    CPC分类号： C12N15/101 ,  C12N15/1006 ,  Y10T436/143333 ,  Y10T436/255

    摘要： The present invention is related to a method for isolating a target nucleic acid from a sample comprising said target nucleic acid, comprising the steps of mixing a sample containing said target nucleic acid with a binding solution and a nucleic acid binding matrix, adsorbing at least part of said target nucleic acid to said nucleic acid binding matrix, wherein said nucleic acid binding matrix is treated simultaneously or has been previously treated with at least one compound comprising a metal substance selected from the group consisting of metals of the main groups 13 to 16, semimetals and transition metals for reducing non-target nucleic acid contaminations, Furthermore, respective kits and reagents are provided with the teaching of the present invention.