公开(公告)号：EP2262898B1

公开(公告)日：2018-01-24

申请号：EP09718915.3

申请日：2009-03-06

申请人： Regeneron Pharmaceuticals, Inc.

发明人： POUEYMIROU, William ,  DECHIARA, Thomas, M. ,  AUERBACH, Wojtek ,  ECONOMIDES, Aris, N. ,  GALE, Nicholas, W. ,  FRENDEWEY, David ,  VALENZUELA,David, M.

IPC分类号： A01K67/027 ,  C12N15/85 ,  C12N15/90 ,  C12N5/10 ,  A61D19/00 ,  C12N5/16

CPC分类号： A01K67/0271 ,  A01K67/0275 ,  A01K2207/12 ,  A01K2217/00 ,  A01K2217/05 ,  A01K2217/07 ,  A01K2217/15 ,  A01K2217/203 ,  A01K2217/30 ,  A01K2227/105 ,  A01K2267/02 ,  C12N5/16 ,  C12N15/8509 ,  C12N15/907 ,  C12N2510/00 ,  C12N2517/02

摘要： Genetically modified mice and nucleic acid constructs for making the genetically modified mice are described. A first mouse having a gene encoding an activator (such as a Cre recombinase) operably linked to a developmentally-regulated promoter (such as a Nanog promoter) is provided. A second mouse having a toxic responder gene (such as a gene encoding diphtheria toxin A) is provided, where the toxic gene is expressed only in the presence of an activator, Embryos from a mating of the first and the second mouse are provided as host embryos suitable for generating mice from donor cells introduced into the host embryos. Ablating the ICM of a mouse embryo physically, chemically, or genetically is described, as well as making F0 generation mice that are substantially or in full derived from donor cells, employing a host mouse embryo with an ablated or nonproliferating ICM.

公开(公告)号：EP2262898A1

公开(公告)日：2010-12-22

申请号：EP09718915.3

申请日：2009-03-06

申请人： Regeneron Pharmaceuticals, Inc.

发明人： POUEYMIROU, William ,  DECHIARA, Thomas, M. ,  AUERBACH, Wojtek ,  ECONOMIDES, Aris, N. ,  GALE, Nicholas, W. ,  FRENDEWEY, David ,  VALENZUELA,David, M.

IPC分类号： C12N15/85 ,  C12N15/90 ,  A61D19/00 ,  A01K67/027 ,  C12N5/10 ,  C12N5/06

CPC分类号： A01K67/0271 ,  A01K67/0275 ,  A01K2207/12 ,  A01K2217/00 ,  A01K2217/05 ,  A01K2217/07 ,  A01K2217/15 ,  A01K2217/203 ,  A01K2217/30 ,  A01K2227/105 ,  A01K2267/02 ,  C12N5/16 ,  C12N15/8509 ,  C12N15/907 ,  C12N2510/00 ,  C12N2517/02

摘要： Genetically modified mice and nucleic acid constructs for making the genetically modified mice are described. A first mouse having a gene encoding an activator (such as a Cre recombinase) operably linked to a developmentally-regulated promoter (such as a Nanog promoter) is provided. A second mouse having a toxic responder gene (such as a gene encoding diphtheria toxin A) is provided, where the toxic gene is expressed only in the presence of an activator, Embryos from a mating of the first and the second mouse are provided as host embryos suitable for generating mice from donor cells introduced into the host embryos. Ablating the ICM of a mouse embryo physically, chemically, or genetically is described, as well as making F0 generation mice that are substantially or in full derived from donor cells, employing a host mouse embryo with an ablated or nonproliferating ICM.