利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

65 条记录 (耗时 0.038 秒)

    PROTECTED GUIDE RNAS (PGRNAS)
    3.
    发明公开
    PROTECTED GUIDE RNAS (PGRNAS) 审中-公开
    受保护的指导RNAS（PGRNAS）

    公开(公告)号：EP3230451A1

    公开(公告)日：2017-10-18

    申请号：EP15819996.8

    申请日：2015-12-11

    申请人： The Broad Institute Inc. ,  Massachusetts Institute of Technology ,  President and Fellows of Harvard College

    发明人： ZHANG, Feng ,  ABUDAYYEH, Omar, O. ,  DAHLMAN, James, E. ,  HSU, Patrick ,  SCOTT, David, A.

    IPC分类号： C12N15/11

    CPC分类号： C12N15/907 ,  A01K67/0275 ,  A01K2217/07 ,  A01K2227/105 ,  C07K14/4702 ,  C12N9/22 ,  C12N9/96 ,  C12N15/11 ,  C12N15/111 ,  C12N2310/20 ,  C12Y301/21004

    摘要： The invention provides for systems, methods, and compositions for altering expression of target gene sequences and related gene products. Provided are structural information on the Cas protein of the CRISPR-Cas system, use of this information in generating modified components of the CRISPR complex, vectors and vector systems which encode one or more components or modified components of a CRISPR complex, as well as methods for the design and use of such vectors and components. Also provided are methods of directing CRISPR complex formation in eukaryotic cells and methods for utilizing the CRISPR-Cas system. In particular the present invention comprehends optimized functional CRISPR-Cas enzyme systems, wherein the guide sequence is modified by secondary structure to increase the specificity of the CRISPR-Cas system and whereby the secondary structure can protect against exonuclease activity and allow for 5′ additions to the guide sequence.

    摘要翻译： 本发明提供了用于改变靶基因序列和相关基因产物表达的系统，方法和组合物。 提供了关于CRISPR-Cas系统的Cas蛋白的结构信息，使用该信息产生CRISPR复合物的修饰组分，编码CRISPR复合物的一种或多种组分或修饰组分的载体和载体系统，以及方法 用于这些载体和组件的设计和使用。 还提供了指导真核细胞中CRISPR复合物形成的方法和利用CRISPR-Cas系统的方法。 特别地，本发明包括优化的功能性CRISPR-Cas酶系统，其中指导序列通过二级结构修饰以增加CRISPR-Cas系统的特异性，并且其中二级结构可以保护免受外切核酸酶活性，并允许5'加入 指导顺序。

    PROTEIN HAVING NUCLEASE ACTIVITY, FUSION PROTEINS AND USES THEREOF
    8.
    发明公开
    PROTEIN HAVING NUCLEASE ACTIVITY, FUSION PROTEINS AND USES THEREOF 审中-公开
    蛋白质麻醉剂NUKLEASE-AKTIVITÄT，FUSIONSPROTEINE UND DEREN VERWENDUNGEN

    公开(公告)号：EP2718440A1

    公开(公告)日：2014-04-16

    申请号：EP12725813.5

    申请日：2012-06-06

    申请人： Helmholtz Zentrum München Deutsches Forschungszentrum für Gesundheit und Umwelt (GmbH)

    发明人： KÜHN, Ralf

    IPC分类号： C12N15/62 ,  C12N15/82 ,  C12N15/85 ,  A01K67/027 ,  C07K14/415

    CPC分类号： C12N15/907 ,  A01K67/0275 ,  A01K2207/05 ,  A01K2217/07 ,  A01K2217/075 ,  A01K2227/105 ,  A01K2267/03 ,  C07K14/195 ,  C07K2319/80 ,  C12N9/16 ,  C12N9/22 ,  C12N15/62 ,  C12N15/8509 ,  C12N2800/80 ,  C12N2999/007 ,  C12Y301/21004

    摘要： The present invention relates to a nucleic acid molecule encoding (I) a polypeptide having the activity of an endonuclease, which is (a) a nucleic acid molecule encoding a polypeptide comprising or consisting of the amino acid sequence of SEQ ID NO: 1; (b) a nucleic acid molecule comprising or consisting of the nucleotide sequence of SEQ ID NO: 2; (c) a nucleic acid molecule encoding an endonuclease, the amino acid sequence of which is at least 70% identical to the amino acid sequence of SEQ ID NO: 1; (d) a nucleic acid molecule comprising or consisting of a nucleotide sequence which is at least 50% identical to the nucleotide sequence of SEQ ID NO: 2; (e) a nucleic acid molecule which is degenerate with respect to the nucleic acid molecule of (d); or (f) a nucleic acid molecule corresponding to the nucleic acid molecule of any one of (a) to (e) wherein T is replaced by U; (II) a fragment of the polypeptide of (I) having the activity of an endonuclease. Also, the present invention relates to a vector comprising the nucleic acid molecule and a protein encoded by said nucleic acid molecule. Further, the invention relates to a method of modifying the genome of a eukaryotic cell and a method of producing a non-human vertebrate or mammal.

    摘要翻译： 本发明涉及编码（I）具有内切核酸酶活性的多肽的核酸分子，其是（a）编码包含或由SEQ ID NO：1的氨基酸序列组成的多肽的核酸分子; （b）包含SEQ ID NO：2的核苷酸序列或由其组成的核酸分子; （c）编码内切核酸酶的核酸分子，其氨基酸序列与SEQ ID NO：1的氨基酸序列至少70％相同; （d）包含与SEQ ID NO：2的核苷酸序列至少50％相同的核苷酸序列或由其组成的核酸分子; （e）相对于（d）的核酸分子简并的核酸分子; 或（f）对应于（a）〜（e）中任一项所述的核酸分子的核酸分子，其中T被U代替; （II）具有内切核酸酶活性的（I）多肽的片段。 此外，本发明涉及包含核酸分子和由所述核酸分子编码的蛋白质的载体。 此外，本发明涉及一种修饰真核细胞的基因组的方法和一种生产非人脊椎动物或哺乳动物的方法。