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1.发明公开FUSION PROTEINS COMPRISING IMMUNOGLOBULIN CONSTANT DOMAIN-DERIVED SCAFFOLDS 审中-公开具有恒定关断免疫球蛋白的框架融合蛋白
公开(公告)号：EP2812432A1
公开(公告)日：2014-12-17
申请号：EP13746683.5
申请日：2013-02-08
发明人： BRAMHILL, David , GEHLSEN, Kurt
CPC分类号： C12N15/815 , C07K16/283 , C07K16/46 , C07K2317/21 , C07K2317/524 , C07K2317/94 , C07K2318/20 , C07K2319/00 , C07K2319/31 , C07K2319/70 , C12P21/00
摘要： This disclosure features fusion proteins comprising a base protein linked to or incorporated in a CH2 scaffold of IgG. The CH2 scaffold can derive from the macaque CH2 domain of IgG. The fusion proteins can effectively bind a single or multiple targets, and can be engineered to regulate effector functions as desired. The fusion proteins can have an increased serum half-life, solubility, stability, protease resistance, and/or expression as compared to the scaffolds alone and/or as compared to the base protein alone. This disclosure also features fusion proteins comprising a base protein, a CH2 scaffold and a discrete polyethylene glycol (dPEG) linked to the scaffold via a serine, tyrosine, cysteine, lysine, or a glycosylation site of the scaffold. This disclosure additionally features scaffolds linked to a discrete polyethylene glycol (dPEG) via a serine, tyrosine, cysteine, or lysine of the scaffolds or a glycosylation site of the scaffold.
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2.发明公开VIABLE GRAM NEGATIVE BACTERIA WITH REDUCED PROTEOLYTIC ACTIVITY LACKING OUTER MEMBRANE AGONISTS OF TLR4/MD-2 有权具有降低的活性蛋白水解LIFE ELIGIBLE革兰氏阴性细菌而不外膜激动剂TLR4 OF / MD-2
公开(公告)号：EP2753688A1
公开(公告)日：2014-07-16
申请号：EP12829962.5
申请日：2012-09-07
发明人： BRAMHILL, David , MAMAT,Uwe
IPC分类号： C12N1/21 , C07K14/245 , C12Q1/68 , C40B40/02
摘要： Viable Gram-negative bacteria or components thereof comprising outer membranes that substantially lack a ligand, such as Lipid A or 6-acyl lipidpolysaccharide, that acts as an agonist of TLR4/MD-2. The bacteria may comprise reduced activity of arabinose-5-phosphate isomerases and one or more suppressor mutations, for example in a transporter thereby increasing the transporter's capacity to transport lipid IVA or in membrane protein YhjD. One or more genes (e.g., lpxL, lpxM, pagP, lpxP, and/or eptA) may be substantially deleted and/or one or more enzymes (e.g., LpxL, LpxM, PagP, LpxP, and/or EptA) may be substantially inactive. The bacteria may be competent to take up extracellular DNA, may be donor bacteria, or may be members of a library. The present invention also features methods of creating and utilizing such bacteria.
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公开(公告)号：EP2753688B1
公开(公告)日：2018-06-20
申请号：EP12829962.5
申请日：2012-09-07
发明人： BRAMHILL, David , MAMAT, Uwe
IPC分类号： C12N1/21 , C07K14/245 , C12Q1/68 , C40B40/02
摘要： Viable Gram-negative bacteria or components thereof comprising outer membranes that substantially lack a ligand, such as Lipid A or 6-acyl lipidpolysaccharide, that acts as an agonist of TLR4/MD-2. The bacteria may comprise reduced activity of arabinose-5-phosphate isomerases and one or more suppressor mutations, for example in a transporter thereby increasing the transporter's capacity to transport lipid IVA or in membrane protein YhjD. One or more genes (e.g., lpxL, lpxM, pagP, lpxP, and/or eptA) may be substantially deleted and/or one or more enzymes (e.g., LpxL, LpxM, PagP, LpxP, and/or EptA) may be substantially inactive. The bacteria may be competent to take up extracellular DNA, may be donor bacteria, or may be members of a library. The present invention also features methods of creating and utilizing such bacteria.
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4.发明公开CH2 DOMAIN TEMPLATE MOLECULES DERIVED FROM RATIONAL GRAFTING OF DONOR LOOPS ONTO CH2 SCAFFOLDS 审中-公开通过定期接枝DONATORSCHLEIFEN对CH2-CH2-GERÜSTEWON域模板分子RATIO
公开(公告)号：EP2672995A2
公开(公告)日：2013-12-18
申请号：EP12744675.5
申请日：2012-02-10
IPC分类号： A61K39/395
CPC分类号： C07K16/461 , C07K16/005 , C07K16/42 , C07K2317/524 , C07K2317/92 , C07K2317/94 , C40B30/04 , G01N33/6854
摘要： Novel CH2 domain template molecules wherein donor loops from a database of domains are transferred to a CH2 domain scaffold. At least one or up to three loops from a donor are transferred to the CH2 domain. The donor loops may be chosen based on length, e.g., the donor loop may have a length that is similar to that of a structural loop in the CH2 domain scaffold.
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5.发明授权
公开(公告)号：EP2672995B1
公开(公告)日：2019-01-02
申请号：EP12744675.5
申请日：2012-02-10
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