公开(公告)号：EP1612264A1

公开(公告)日：2006-01-04

申请号：EP04721319.4

申请日：2004-03-17

申请人： Research Foundation Itsuu Laboratory

发明人： ASASHIMA, Makoto ,  HAMAZAKI, Tatsuo ,  KAGECHIKA, Hiroyuki ,  SHUDO, Koichi

IPC分类号： C12N5/06

CPC分类号： C12N5/0062 ,  C12N2501/385 ,  C12N2501/999 ,  C12N2506/00

摘要： A method for forming an organ and/or tissue from undifferentiated cells derived from a vertebrate animal in vitro, which comprises the step of culturing the undifferentiated cells derived from a vertebrate animal in the presence of a retinoic acid X receptor ligand (e.g., a retinoic acid X receptor agonist or antagonist), and a method for forming a pancreas from undifferentiated cells derived from a vertebrate animal in vitro or a method for forming a tissue having morphology and function of a pancreas from undifferentiated cells derived from a vertebrate in vitro, which comprises the step of culturing the undifferentiated cells derived from a vertebrate animal in the presence of a retinoic acid receptor ligand, together with activin, that does not substantially bind to the retinoic acid receptor subtype γ.

摘要翻译： 一种由体外来源于脊椎动物的未分化细胞形成器官和/或组织的方法，该方法包括以下步骤：在视黄酸X受体配体（例如，视黄酸）存在下培养源自脊椎动物的未分化细胞 酸X受体激动剂或拮抗剂）以及由体外来源于脊椎动物的未分化细胞形成胰腺的方法，或体外来源于脊椎动物的未分化细胞形成具有胰腺形态和功能的组织的方法， 包括在视黄酸受体配体存在的情况下培养源自脊椎动物的未分化细胞以及基本上不与视黄酸受体亚型γ结合的活化素的步骤。