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    METHOD FOR THE PRODUCTION OF POLYPEPTIDES
    1.
    发明公开
    METHOD FOR THE PRODUCTION OF POLYPEPTIDES 审中-公开

    公开(公告)号：EP3517621A1

    公开(公告)日：2019-07-31

    申请号：EP19161593.9

    申请日：2013-03-18

    申请人： Richter Gedeon Nyrt.

    发明人： FELFÖLDI, Ferenc ,  OLASZ, Katalin ,  KOZMA, József

    IPC分类号： C12P21/02 ,  C07K14/535

    摘要： New methods for the production of recombinant polypeptides from inclusion bodies are disclosed. Modulation of the cell culture conditions positively affects the yield of the recombinant polypeptide in active form. In one aspect, the methods comprise (a) cultivating a host cell at a first temperature, the host cell comprising a nucleic acid encoding a recombinant polypeptide, (b) lowering the cultivation temperature from the first temperature to a second temperature, and (c) cultivating the host cell at the second temperature.

    METHOD FOR THE PRODUCTION OF POLYPEPTIDES
    3.
    发明公开
    METHOD FOR THE PRODUCTION OF POLYPEPTIDES 审中-公开
    生产多肽的方法

    公开(公告)号：EP2828396A2

    公开(公告)日：2015-01-28

    申请号：EP13709469.4

    申请日：2013-03-18

    申请人： Richter Gedeon Nyrt.

    发明人： FELFÖLDI, Ferenc ,  OLASZ, Katalin ,  KOZMA, József

    IPC分类号： C12P21/02 ,  C07K14/535

    CPC分类号： C07K14/535 ,  C12P21/02

    摘要： New methods for the production of recombinant polypeptides from inclusion bodies are disclosed. Modulation of the cell culture conditions positively affects the yield of the recombinant polypeptide in active form. In one aspect, the methods comprise (a) cultivating a host cell at a first temperature, the host cell comprising a nucleic acid encoding a recombinant polypeptide, (b) lowering the cultivation temperature from the first temperature to a second temperature, and (c) cultivating the host cell at the second temperature.

    摘要翻译： 公开了从包涵体产生重组多肽的新方法。 细胞培养条件的调节正面影响活性形式的重组多肽的产量。 在一个方面，所述方法包括（a）在第一温度下培养宿主细胞，所述宿主细胞包含编码重组多肽的核酸，（b）将培养温度从第一温度降低至第二温度，以及（c ）在第二温度下培养宿主细胞。