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公开(公告)号:EP1466018B1
公开(公告)日:2007-10-03
申请号:EP03729220.8
申请日:2003-01-04
发明人: PINSL-OBER, Judith , WENZIG, Peter , WEINDEL, Kurt , BARTL, Knut , SCHOENBRUNNER, Ralf , MALHOTRA, Khushbeer , O'DONNELL, Patrick , KYGER, Erich
CPC分类号: C12N15/1006 , C12Q1/6806 , C12Q2547/101 , C12Q2531/113
摘要: This invention relates to a method for the isolation of a target nucleic acid using a material with an unmodified silica surface and subsequent amplification of the target nucleic acid in the presence of the material with an unmodified silica surface. The method is preferably carried out as an automated process preferably in a high throughput format. The method is preferably used in diagnostics.
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2.
公开(公告)号:EP2521779B1
公开(公告)日:2014-12-10
申请号:EP11701607.1
申请日:2011-01-05
发明人: JOHNSON, Jenny, A. , KYGER, Erich
CPC分类号: C12N15/1006 , C12N15/1013 , C12Q1/6834 , C12Q2527/125 , C12Q2563/143
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公开(公告)号:EP1466018B2
公开(公告)日:2015-08-12
申请号:EP03729220.8
申请日:2003-01-04
发明人: PINSL-OBER, Judith , WENZIG, Peter , WEINDEL, Kurt , BARTL, Knut , SCHOENBRUNNER, Ralf , MALHOTRA, Khushbeer , O'DONNELL, Patrick , KYGER, Erich
CPC分类号: C12N15/1006 , C12Q1/6806 , C12Q2547/101 , C12Q2531/113
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4.
公开(公告)号:EP2521779A1
公开(公告)日:2012-11-14
申请号:EP11701607.1
申请日:2011-01-05
发明人: JOHNSON, Jenny, A. , KYGER, Erich
CPC分类号: C12N15/1006 , C12N15/1013 , C12Q1/6834 , C12Q2527/125 , C12Q2563/143
摘要: The present invention is a method of separating nucleic acids using a solid phase capable of binding nucleic acids, such as magnetic glass particles, where binding of nucleic acids to the solid phase is enhanced by the presence of an ethylene-amine compound. The invention further includes a reaction mixture for isolating nucleic acids containing an ethylene-amine compound and kits for carrying out the method.
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公开(公告)号:EP1466018A2
公开(公告)日:2004-10-13
申请号:EP03729220.8
申请日:2003-01-04
发明人: PINSL-OBER, Judith , WENZIG, Peter , WEINDEL, Kurt , BARTL, Knut , SCHOENBRUNNER, Ralf , MALHOTRA, Khushbeer , O'DONNELL, Patrick , KYGER, Erich
CPC分类号: C12N15/1006 , C12Q1/6806 , C12Q2547/101 , C12Q2531/113
摘要: This invention relates to a method for the isolation of a target nucleic acid using a material with an unmodified silica surface and subsequent amplification of the target nucleic acid in the presence of the material with an unmodified silica surface. The method is preferably carried out as an automated process preferably in a high throughput format. The method is preferably used in diagnostics.
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