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1.发明公开DETERMINATION OF KIR HAPLOTYPES ASSOCIATED WITH DISEASE 有权BESTIMMUNG VON KIR-HAPLOTYPEN DURCH AMPLIFATIONATION DER EXONS
公开(公告)号：EP2480683A1
公开(公告)日：2012-08-01
申请号：EP10757165.5
申请日：2010-09-17
发明人： BENTLEY, Gordon , ERLICH, Henry A. , GOODRIDGE, Damian , LADNER, Martha , TRACHTENBERG, Elizabeth
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6883 , C12Q1/6881 , C12Q2600/156 , C12Q2600/16 , C12Q2600/172 , G06F19/00 , Y02A90/26
摘要： Disclosed is a method of determining KIR genotypes for one or more individuals in parallel, the method comprising: for each individual, amplifying the polymorphic exon sequences of the KIR genes, pooling the KIR amplicons, performing emulsion PCR followed by pyrosequencing in parallel to determine all the amplicon sequences present in the individual to determine which KIR alleles are present in the individual.
摘要翻译： 公开了一种平行测定一个或多个个体的KIR基因型的方法，所述方法包括：对于每个个体，扩增KIR基因的多态性外显子序列，汇集KIR扩增子，进行乳液PCR，然后并行进行焦磷酸测序以确定所有 存在于个体中的扩增子序列以确定个体中存在哪些KIR等位基因。
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公开(公告)号：EP2480683B1
公开(公告)日：2017-11-29
申请号：EP10757165.5
申请日：2010-09-17
发明人： BENTLEY, Gordon , ERLICH, Henry A. , GOODRIDGE, Damian , LADNER, Martha , TRACHTENBERG, Elizabeth
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6883 , C12Q1/6881 , C12Q2600/156 , C12Q2600/16 , C12Q2600/172 , G06F19/00 , Y02A90/26
摘要： Disclosed is a method of determining KIR genotypes for one or more individuals in parallel, the method comprising: for each individual, amplifying the polymorphic exon sequences of the KIR genes, pooling the KIR amplicons, performing emulsion PCR followed by pyrosequencing in parallel to determine all the amplicon sequences present in the individual to determine which KIR alleles are present in the individual.
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